Methodology to Study NF-κB/RelA Ubiquitination in Vivo

Haiying Li, Petro Starokadomskyy, Ezra Burstein

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nuclear factor-kappa B (NF-κB) is a family of transcription factors that regulate immune responses, cell proliferation, differentiation, and survival. Activity of the NF-κΒ pathway on a cellular level is tightly controlled through various mechanisms, one of which is the ubiquitin-dependent degradation of chromatinbound NF-κB subunits. In general, the ubiquitination of NF-κB regulates the duration of gene transcription activated in response to infl ammatory signals. In this article, we present protocols to examine the in vivo ubiquitination status of RelA, a critical protein of the NF-κB family.

Original languageEnglish (US)
Pages (from-to)371-381
Number of pages11
JournalMethods in Molecular Biology
Volume1280
DOIs
StatePublished - 2015

Fingerprint

NF-kappa B
Ubiquitination
Ubiquitin
Cell Differentiation
Cell Survival
Transcription Factors
Cell Proliferation
Genes
Proteins

Keywords

  • Immunoprecipitation
  • IκB
  • NF-κB
  • RelA
  • Ubiquitin
  • Ubiquitination

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Methodology to Study NF-κB/RelA Ubiquitination in Vivo. / Li, Haiying; Starokadomskyy, Petro; Burstein, Ezra.

In: Methods in Molecular Biology, Vol. 1280, 2015, p. 371-381.

Research output: Contribution to journalArticle

Li, Haiying ; Starokadomskyy, Petro ; Burstein, Ezra. / Methodology to Study NF-κB/RelA Ubiquitination in Vivo. In: Methods in Molecular Biology. 2015 ; Vol. 1280. pp. 371-381.
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