TY - JOUR
T1 - Methodology to Study NF-κB/RelA Ubiquitination in Vivo
AU - Li, Haiying
AU - Starokadomskyy, Petro
AU - Burstein, Ezra
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Nuclear factor-kappa B (NF-κB) is a family of transcription factors that regulate immune responses, cell proliferation, differentiation, and survival. Activity of the NF-κΒ pathway on a cellular level is tightly controlled through various mechanisms, one of which is the ubiquitin-dependent degradation of chromatinbound NF-κB subunits. In general, the ubiquitination of NF-κB regulates the duration of gene transcription activated in response to infl ammatory signals. In this article, we present protocols to examine the in vivo ubiquitination status of RelA, a critical protein of the NF-κB family.
AB - Nuclear factor-kappa B (NF-κB) is a family of transcription factors that regulate immune responses, cell proliferation, differentiation, and survival. Activity of the NF-κΒ pathway on a cellular level is tightly controlled through various mechanisms, one of which is the ubiquitin-dependent degradation of chromatinbound NF-κB subunits. In general, the ubiquitination of NF-κB regulates the duration of gene transcription activated in response to infl ammatory signals. In this article, we present protocols to examine the in vivo ubiquitination status of RelA, a critical protein of the NF-κB family.
KW - Immunoprecipitation
KW - IκB
KW - NF-κB
KW - RelA
KW - Ubiquitin
KW - Ubiquitination
UR - http://www.scopus.com/inward/record.url?scp=84924356141&partnerID=8YFLogxK
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U2 - 10.1007/978-1-4939-2422-6_22
DO - 10.1007/978-1-4939-2422-6_22
M3 - Article
C2 - 25736761
AN - SCOPUS:84924356141
VL - 1280
SP - 371
EP - 381
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -