Methodology to Study NF-κB/RelA Ubiquitination in Vivo

Haiying Li, Petro Starokadomskyy, Ezra Burstein

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Nuclear factor-kappa B (NF-κB) is a family of transcription factors that regulate immune responses, cell proliferation, differentiation, and survival. Activity of the NF-κΒ pathway on a cellular level is tightly controlled through various mechanisms, one of which is the ubiquitin-dependent degradation of chromatinbound NF-κB subunits. In general, the ubiquitination of NF-κB regulates the duration of gene transcription activated in response to infl ammatory signals. In this article, we present protocols to examine the in vivo ubiquitination status of RelA, a critical protein of the NF-κB family.

Original languageEnglish (US)
Pages (from-to)371-381
Number of pages11
JournalMethods in Molecular Biology
Volume1280
DOIs
Publication statusPublished - 2015

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Keywords

  • Immunoprecipitation
  • IκB
  • NF-κB
  • RelA
  • Ubiquitin
  • Ubiquitination

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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