Methods for gene transfer to the central nervous system

Boris Kantor, Rachel M. Bailey, Keon Wimberly, Sahana N. Kalburgi, Steven J. Gray

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Gene transfer is an increasingly utilized approach for research and clinical applications involving the central nervous system (CNS). Vectors for gene transfer can be as simple as an unmodified plasmid, but more commonly involve complex modifications to viruses to make them suitable gene delivery vehicles. This chapter will explain how tools for CNS gene transfer have been derived from naturally occurring viruses. The current capabilities of plasmid, retroviral, adeno-associated virus, adenovirus, and herpes simplex virus vectors for CNS gene delivery will be described. These include both focal and global CNS gene transfer strategies, with short- or long-term gene expression. As is described in this chapter, an important aspect of any vector is the cis-acting regulatory elements incorporated into the vector genome that control when, where, and how the transgene is expressed.

Original languageEnglish (US)
Pages (from-to)125-197
Number of pages73
JournalAdvances in Genetics
Volume87
DOIs
StatePublished - Jan 1 2014

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Central Nervous System
Genes
Plasmids
Viruses
Dependovirus
Simplexvirus
Transgenes
Adenoviridae
Genome
Gene Expression
Research

Keywords

  • AAV
  • Adenovirus
  • CNS
  • Gene therapy
  • Lentivirus
  • Plasmid
  • Promoter
  • Vector

ASJC Scopus subject areas

  • Genetics

Cite this

Methods for gene transfer to the central nervous system. / Kantor, Boris; Bailey, Rachel M.; Wimberly, Keon; Kalburgi, Sahana N.; Gray, Steven J.

In: Advances in Genetics, Vol. 87, 01.01.2014, p. 125-197.

Research output: Contribution to journalArticle

Kantor, Boris ; Bailey, Rachel M. ; Wimberly, Keon ; Kalburgi, Sahana N. ; Gray, Steven J. / Methods for gene transfer to the central nervous system. In: Advances in Genetics. 2014 ; Vol. 87. pp. 125-197.
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