Methods for renal lineage tracing: In vivo and beyond

Keri A. Drake, Alicia R. Fessler, Thomas J. Carroll

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lineage tracing has resulted in fundamental discoveries in kidney development and disease and remains a powerful technique to study mechanisms of organogenesis, homeostasis, and repair/regeneration. Following decades of research on the cellular and molecular regulation of renal organogenesis, the kidney has become one of the most well-characterized organs, resulting in exciting advancements in pluripotent stem cell differentiation, tissue bioengineering, and the potential for developing novel regenerative therapies for kidney disease. Lineage tracing, or the labeling of progeny cells arising from a single cell or group of cells, allows for spatial and temporal analyses of dynamic in vivo and in vitro processes. As lineage tracing techniques expand across disciplines of developmental biology, stem cell biology, and regenerative medicine, careful experimental design and interpretation, along with an understanding of the basic principles and technical limitations, are essential for utilizing genetically complex lineage tracing models to further understand kidney development and disease.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
EditorsThomas Weimbs
PublisherAcademic Press Inc.
Pages121-143
Number of pages23
ISBN (Print)9780128203354
DOIs
StatePublished - 2019

Publication series

NameMethods in Cell Biology
Volume154
ISSN (Print)0091-679X

Keywords

  • Cre/lox recombination
  • Lineage tracing
  • Reporters

ASJC Scopus subject areas

  • Cell Biology

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    Drake, K. A., Fessler, A. R., & Carroll, T. J. (2019). Methods for renal lineage tracing: In vivo and beyond. In T. Weimbs (Ed.), Methods in Cell Biology (pp. 121-143). (Methods in Cell Biology; Vol. 154). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2019.06.002