Methods for renal lineage tracing: In vivo and beyond

Keri Anne Drake, Alicia R. Fessler, Thomas J Carroll

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lineage tracing has resulted in fundamental discoveries in kidney development and disease and remains a powerful technique to study mechanisms of organogenesis, homeostasis, and repair/regeneration. Following decades of research on the cellular and molecular regulation of renal organogenesis, the kidney has become one of the most well-characterized organs, resulting in exciting advancements in pluripotent stem cell differentiation, tissue bioengineering, and the potential for developing novel regenerative therapies for kidney disease. Lineage tracing, or the labeling of progeny cells arising from a single cell or group of cells, allows for spatial and temporal analyses of dynamic in vivo and in vitro processes. As lineage tracing techniques expand across disciplines of developmental biology, stem cell biology, and regenerative medicine, careful experimental design and interpretation, along with an understanding of the basic principles and technical limitations, are essential for utilizing genetically complex lineage tracing models to further understand kidney development and disease.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X

Fingerprint

Kidney Diseases
Organogenesis
Kidney
Spatio-Temporal Analysis
Developmental Biology
Bioengineering
Pluripotent Stem Cells
Regenerative Medicine
Cell Biology
Regeneration
Cell Differentiation
Homeostasis
Research Design
Stem Cells
Research
Therapeutics

Keywords

  • Cre/lox recombination
  • Lineage tracing
  • Reporters

ASJC Scopus subject areas

  • Cell Biology

Cite this

Drake, K. A., Fessler, A. R., & Carroll, T. J. (2019). Methods for renal lineage tracing: In vivo and beyond. In Methods in Cell Biology (Methods in Cell Biology). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2019.06.002

Methods for renal lineage tracing : In vivo and beyond. / Drake, Keri Anne; Fessler, Alicia R.; Carroll, Thomas J.

Methods in Cell Biology. Academic Press Inc., 2019. (Methods in Cell Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Drake, KA, Fessler, AR & Carroll, TJ 2019, Methods for renal lineage tracing: In vivo and beyond. in Methods in Cell Biology. Methods in Cell Biology, Academic Press Inc. https://doi.org/10.1016/bs.mcb.2019.06.002
Drake KA, Fessler AR, Carroll TJ. Methods for renal lineage tracing: In vivo and beyond. In Methods in Cell Biology. Academic Press Inc. 2019. (Methods in Cell Biology). https://doi.org/10.1016/bs.mcb.2019.06.002
Drake, Keri Anne ; Fessler, Alicia R. ; Carroll, Thomas J. / Methods for renal lineage tracing : In vivo and beyond. Methods in Cell Biology. Academic Press Inc., 2019. (Methods in Cell Biology).
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