Methods for studying the germline of the human parasite schistosoma mansoni

Julie N R Collins, James J. Collins

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Schistosomes are flatworm parasites that claim the lives of more than 200,000 people in poverty-stricken regions every year. Much of the pathology due to infection is the direct result of injury spurred by the parasite’s eggs becoming lodged in host tissues. Thus, asking basic questions about germ cell biology may not only identify novel therapeutic approaches, but could also uncover conserved mechanisms that regulate the germline in diverse metazoa. Here, we detail useful methods for studying the schistosome germline including EdU labeling, whole-mount in situ hybridization, and RNA interference. These methods will hopefully lead to new insights about germline development in the schistosome and facilitate new investigators to begin asking questions about these important and fascinating parasites.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages35-47
Number of pages13
Volume1463
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1463
ISSN (Print)10643745

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Keywords

  • Germ cell
  • Reproduction
  • Schistosome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Collins, J. N. R., & Collins, J. J. (2017). Methods for studying the germline of the human parasite schistosoma mansoni. In Methods in Molecular Biology (Vol. 1463, pp. 35-47). (Methods in Molecular Biology; Vol. 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_2