Methods for studying the germline of the human parasite schistosoma mansoni

Julie N R Collins, James J. Collins

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Schistosomes are flatworm parasites that claim the lives of more than 200,000 people in poverty-stricken regions every year. Much of the pathology due to infection is the direct result of injury spurred by the parasite’s eggs becoming lodged in host tissues. Thus, asking basic questions about germ cell biology may not only identify novel therapeutic approaches, but could also uncover conserved mechanisms that regulate the germline in diverse metazoa. Here, we detail useful methods for studying the schistosome germline including EdU labeling, whole-mount in situ hybridization, and RNA interference. These methods will hopefully lead to new insights about germline development in the schistosome and facilitate new investigators to begin asking questions about these important and fascinating parasites.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages35-47
Number of pages13
Volume1463
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1463
ISSN (Print)10643745

Fingerprint

Schistosoma mansoni
Parasites
Platyhelminths
Poverty
RNA Interference
Germ Cells
Eggs
In Situ Hybridization
Cell Biology
Research Personnel
Pathology
Wounds and Injuries
Infection
Therapeutics

Keywords

  • Germ cell
  • Reproduction
  • Schistosome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Collins, J. N. R., & Collins, J. J. (2017). Methods for studying the germline of the human parasite schistosoma mansoni. In Methods in Molecular Biology (Vol. 1463, pp. 35-47). (Methods in Molecular Biology; Vol. 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_2

Methods for studying the germline of the human parasite schistosoma mansoni. / Collins, Julie N R; Collins, James J.

Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. p. 35-47 (Methods in Molecular Biology; Vol. 1463).

Research output: Chapter in Book/Report/Conference proceedingChapter

Collins, JNR & Collins, JJ 2017, Methods for studying the germline of the human parasite schistosoma mansoni. in Methods in Molecular Biology. vol. 1463, Methods in Molecular Biology, vol. 1463, Humana Press Inc., pp. 35-47. https://doi.org/10.1007/978-1-4939-4017-2_2
Collins JNR, Collins JJ. Methods for studying the germline of the human parasite schistosoma mansoni. In Methods in Molecular Biology. Vol. 1463. Humana Press Inc. 2017. p. 35-47. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-4017-2_2
Collins, Julie N R ; Collins, James J. / Methods for studying the germline of the human parasite schistosoma mansoni. Methods in Molecular Biology. Vol. 1463 Humana Press Inc., 2017. pp. 35-47 (Methods in Molecular Biology).
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