Methods to purify and assay secretory pathway kinases

Vincent S. Tagliabracci, Jianzhong Wen, Junyu Xiao

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Members of the four-jointed and VLK families of secretory pathway kinases appear to be responsible for the phosphorylation of secreted proteins and proteoglycans. These enzymes have been implicated in many biological processes and mutations in several of these kinases cause human diseases. Here, we describe methods to purify and assay two members of the four-jointed family of secretory kinases: the Fam20C protein kinase and the Fam20B proteoglycan kinase.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages197-215
Number of pages19
Volume1496
DOIs
StatePublished - Sep 1 2016

Publication series

NameMethods in Molecular Biology
Volume1496
ISSN (Print)1064-3745

Keywords

  • Amelogenesis imperfect
  • Golgi kinase
  • Hypophosphatemia
  • Raine syndrome
  • Secreted kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Tagliabracci, V. S., Wen, J., & Xiao, J. (2016). Methods to purify and assay secretory pathway kinases. In Methods in Molecular Biology (Vol. 1496, pp. 197-215). (Methods in Molecular Biology; Vol. 1496). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6463-5_16