Mhc class I and non-class I gene organization in the proximal H2-M region of the mouse

Elsy P. Jones, Attila Kumánovics, Masayasu Yoshino, Kirsten Fischer Lindahl

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A bacterial artificial chromosome (BAC) contig was constructed across the proximal part of the H2-M region from the major histocompatibility complex (Mhc) of mouse strain 129 (H2(bc)). The contig is composed of 28 clones that span approximately 1 megabasepair (Mb), from H2-T1 to Mog, and contains three H2-T genes and 18 H2-M genes. We report the fine mapping of the H2-M class I gene cluster, which includes the previously reported M4-M6, the M1 family, the M10 family, and four additional class I genes. All but two of the H2-M class I genes are conserved among haplotypes H2(k), H2b, and H2(bc), and only two genes are found in polymorphic HindIII fragments. Six evolutionarily conserved non-class I genes were mapped to a 180 kilobase interval in the distal part of the class I region in mouse, and their order Znf173-Rfb30-Tctex5-Tctex6-Tctex4-Mog was found conserved between human and mouse. In this Znf173-Mog interval, three mouse class I genes, M6, M4, and M5, which are conserved among haplotypes, occupy the same map position as the human HLA-A class I cluster, which varies among haplotypes and is diverged in sequence from the mouse genes. These results further support the view that class I gene diverge and evolve independently between species.

Original languageEnglish (US)
Pages (from-to)183-195
Number of pages13
JournalImmunogenetics
Volume49
Issue number3
DOIs
StatePublished - Feb 4 1999

Keywords

  • Genomic evolution
  • H2(bc)
  • Major histocompatibility complex
  • Physical mapping
  • Synteny conservation

ASJC Scopus subject areas

  • Immunology
  • Genetics

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