Mice without myoglobin

Daniel J. Garry, George A. Ordway, John N. Lorenz, Nina B. Radford, Eva R. Chin, Robert W. Grange, Rhonda Bassel-Duby, R. Sanders Williams

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

Myoglobin, an intracellular haemoprotein expressed in the heart and oxidative skeletal myofibres of vertebrates, binds molecular oxygen and may facilitate oxygen transport from erythrocytes to mitochondria, thereby maintaining cellular respiration during periods of high physiological demand. Here we show, however, that mice without myoglobin, generated by gene- knockout technology, are fertile and exhibit normal exercise capacity and a normal ventilatory response to low oxygen levels (hypoxia). Heart and soleus muscles from these animals are depigmented, but function normally in standard assays of muscle performance in vitro across a range of work conditions and oxygen availability. These data show that myoglobin is not required to meet the metabolic requirements of pregnancy or exercise in a terrestrial mammal, and raise new questions about oxygen transport and metabolic regulation in working muscles.

Original languageEnglish (US)
Pages (from-to)905-908
Number of pages4
JournalNature
Volume395
Issue number6705
DOIs
StatePublished - Oct 29 1998

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Myoglobin
Oxygen
Cell Respiration
Muscles
Gene Knockout Techniques
Vertebrates
Mammals
Myocardium
Mitochondria
Skeletal Muscle
Erythrocytes
Technology
Pregnancy

ASJC Scopus subject areas

  • General

Cite this

Garry, D. J., Ordway, G. A., Lorenz, J. N., Radford, N. B., Chin, E. R., Grange, R. W., ... Williams, R. S. (1998). Mice without myoglobin. Nature, 395(6705), 905-908. https://doi.org/10.1038/27681

Mice without myoglobin. / Garry, Daniel J.; Ordway, George A.; Lorenz, John N.; Radford, Nina B.; Chin, Eva R.; Grange, Robert W.; Bassel-Duby, Rhonda; Williams, R. Sanders.

In: Nature, Vol. 395, No. 6705, 29.10.1998, p. 905-908.

Research output: Contribution to journalArticle

Garry, DJ, Ordway, GA, Lorenz, JN, Radford, NB, Chin, ER, Grange, RW, Bassel-Duby, R & Williams, RS 1998, 'Mice without myoglobin', Nature, vol. 395, no. 6705, pp. 905-908. https://doi.org/10.1038/27681
Garry DJ, Ordway GA, Lorenz JN, Radford NB, Chin ER, Grange RW et al. Mice without myoglobin. Nature. 1998 Oct 29;395(6705):905-908. https://doi.org/10.1038/27681
Garry, Daniel J. ; Ordway, George A. ; Lorenz, John N. ; Radford, Nina B. ; Chin, Eva R. ; Grange, Robert W. ; Bassel-Duby, Rhonda ; Williams, R. Sanders. / Mice without myoglobin. In: Nature. 1998 ; Vol. 395, No. 6705. pp. 905-908.
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