Micro-environment causes reversible changes in DNA methylation and mRNA expression profiles in patient-derived glioma stem cells

Mehmet Baysan, Kevin Woolard, Serdar Bozdag, Gregory Riddick, Svetlana Kotliarova, Margaret C. Cam, Galina I. Belova, Susie Ahn, Wei Zhang, Hua Song, Jennifer Walling, Holly Stevenson, Paul Meltzer, Howard A. Fine

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In vitro and in vivo models are widely used in cancer research. Characterizing the similarities and differences between a patient's tumor and corresponding in vitro and in vivo models is important for understanding the potential clinical relevance of experimental data generated with these models. Towards this aim, we analyzed the genomic aberrations, DNA methylation and transcriptome profiles of five parental tumors and their matched in vitro isolated glioma stem cell (GSC) lines and xenografts generated from these same GSCs using high-resolution platforms. We observed that the methylation and transcriptome profiles of in vitro GSCs were significantly different from their corresponding xenografts, which were actually more similar to their original parental tumors. This points to the potentially critical role of the brain microenvironment in influencing methylation and transcriptional patterns of GSCs. Consistent with this possibility, ex vivo cultured GSCs isolated from xenografts showed a tendency to return to their initial in vitro states even after a short time in culture, supporting a rapid dynamic adaptation to the in vitro microenvironment. These results show that methylation and transcriptome profiles are highly dependent on the microenvironment and growth in orthotopic sites partially reverse the changes caused by in vitro culturing.

Original languageEnglish (US)
Article numbere94045
JournalPLoS One
Volume9
Issue number4
DOIs
StatePublished - Apr 11 2014

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Methylation
DNA methylation
DNA Methylation
Stem cells
Heterografts
Glioma
stem cells
Tumors
Stem Cells
transcriptome
methylation
Messenger RNA
neoplasms
Transcriptome
Aberrations
Brain
Neoplasms
cell lines
brain
genomics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Baysan, M., Woolard, K., Bozdag, S., Riddick, G., Kotliarova, S., Cam, M. C., ... Fine, H. A. (2014). Micro-environment causes reversible changes in DNA methylation and mRNA expression profiles in patient-derived glioma stem cells. PLoS One, 9(4), [e94045]. https://doi.org/10.1371/journal.pone.0094045

Micro-environment causes reversible changes in DNA methylation and mRNA expression profiles in patient-derived glioma stem cells. / Baysan, Mehmet; Woolard, Kevin; Bozdag, Serdar; Riddick, Gregory; Kotliarova, Svetlana; Cam, Margaret C.; Belova, Galina I.; Ahn, Susie; Zhang, Wei; Song, Hua; Walling, Jennifer; Stevenson, Holly; Meltzer, Paul; Fine, Howard A.

In: PLoS One, Vol. 9, No. 4, e94045, 11.04.2014.

Research output: Contribution to journalArticle

Baysan, M, Woolard, K, Bozdag, S, Riddick, G, Kotliarova, S, Cam, MC, Belova, GI, Ahn, S, Zhang, W, Song, H, Walling, J, Stevenson, H, Meltzer, P & Fine, HA 2014, 'Micro-environment causes reversible changes in DNA methylation and mRNA expression profiles in patient-derived glioma stem cells', PLoS One, vol. 9, no. 4, e94045. https://doi.org/10.1371/journal.pone.0094045
Baysan, Mehmet ; Woolard, Kevin ; Bozdag, Serdar ; Riddick, Gregory ; Kotliarova, Svetlana ; Cam, Margaret C. ; Belova, Galina I. ; Ahn, Susie ; Zhang, Wei ; Song, Hua ; Walling, Jennifer ; Stevenson, Holly ; Meltzer, Paul ; Fine, Howard A. / Micro-environment causes reversible changes in DNA methylation and mRNA expression profiles in patient-derived glioma stem cells. In: PLoS One. 2014 ; Vol. 9, No. 4.
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