Microfluidic flow cytometer for multiparameter screening of fluorophore photophysics

Kevin M. Dean, Lloyd M. Davis, Jennifer L. Lubbeck, Premashis Manna, Amy E. Palmer, Ralph Jimenez

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a microfluidic cytometer that sorts mammalian or yeast cells by laser force deflection following real-time multibeam, multiparameter fluorescence measurements, including photobleaching, lifetime and expression level, of the intrinsic fluorophores within each cell.

Original languageEnglish (US)
Title of host publicationCLEO
Subtitle of host publicationScience and Innovations, CLEO_SI 2014
PublisherOptical Society of American (OSA)
ISBN (Print)9781557529992
StatePublished - Jan 1 2014
Externally publishedYes
EventCLEO: Science and Innovations, CLEO_SI 2014 - San Jose, CA, United States
Duration: Jun 8 2014Jun 13 2014

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceCLEO: Science and Innovations, CLEO_SI 2014
CountryUnited States
CitySan Jose, CA
Period6/8/146/13/14

Fingerprint

Photobleaching
Fluorophores
yeast
Microfluidics
Yeast
deflection
Screening
screening
Fluorescence
Cells
life (durability)
fluorescence
Lasers
cells
lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation

Cite this

Dean, K. M., Davis, L. M., Lubbeck, J. L., Manna, P., Palmer, A. E., & Jimenez, R. (2014). Microfluidic flow cytometer for multiparameter screening of fluorophore photophysics. In CLEO: Science and Innovations, CLEO_SI 2014 (Optics InfoBase Conference Papers). Optical Society of American (OSA).

Microfluidic flow cytometer for multiparameter screening of fluorophore photophysics. / Dean, Kevin M.; Davis, Lloyd M.; Lubbeck, Jennifer L.; Manna, Premashis; Palmer, Amy E.; Jimenez, Ralph.

CLEO: Science and Innovations, CLEO_SI 2014. Optical Society of American (OSA), 2014. (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Dean, KM, Davis, LM, Lubbeck, JL, Manna, P, Palmer, AE & Jimenez, R 2014, Microfluidic flow cytometer for multiparameter screening of fluorophore photophysics. in CLEO: Science and Innovations, CLEO_SI 2014. Optics InfoBase Conference Papers, Optical Society of American (OSA), CLEO: Science and Innovations, CLEO_SI 2014, San Jose, CA, United States, 6/8/14.
Dean KM, Davis LM, Lubbeck JL, Manna P, Palmer AE, Jimenez R. Microfluidic flow cytometer for multiparameter screening of fluorophore photophysics. In CLEO: Science and Innovations, CLEO_SI 2014. Optical Society of American (OSA). 2014. (Optics InfoBase Conference Papers).
Dean, Kevin M. ; Davis, Lloyd M. ; Lubbeck, Jennifer L. ; Manna, Premashis ; Palmer, Amy E. ; Jimenez, Ralph. / Microfluidic flow cytometer for multiparameter screening of fluorophore photophysics. CLEO: Science and Innovations, CLEO_SI 2014. Optical Society of American (OSA), 2014. (Optics InfoBase Conference Papers).
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