Micronucleation of human somatic cells with colcemid

Andrew H. Crenshaw, Jerry W. Shay, Leonard R. Murrell

Research output: Contribution to journalArticle

Abstract

A method is described for the production with colcemid of micronucleate cells from normal human fibroblasts (Detroit 550) and human aneuploid D98/AH2 cells. The colcemid dose is titrated to a concentration that prevents cellular attachment to, and spreading on, the substrate following attempted mitosis. Washing out the drug with growth medium followed by incubation in drug-free medium for 12 to 24 h produces micronucleate cells. The percentage of micronucleation obtainable is 50 to 70% by treatment with 0.1 ug/ml colcemid for 24 h followed by incubation of floating blocked mitotic cells in drug-free growth medium.

Original languageEnglish (US)
Pages (from-to)185-187
Number of pages3
JournalJournal of Tissue Culture Methods
Volume8
Issue number4
DOIs
StatePublished - Dec 1983

Keywords

  • colcemid
  • colchicine
  • human fibroblasts
  • micronucleation

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

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