Abstract
Vascular endothelial growth factor-C (VEGF-C) is a secreted growth factor essential for lymphangiogenesis. VEGF-C functions in both physiological and pathological lymphangiogenesis, particularly in tumor metastasis, making it an attractive therapeutic target. Members of two families of cell surface receptors transduce VEGF-C signals: neuropilin-2 (Nrp2) and VEGF-receptor (VEGFR)-2/3. Nrp2 is a promising target for inhibition because it is highly expressed in lymphatic vessels. Here we describe a microplate-based assay for discovery of VEGF-C/Nrp2 inhibitors. We optimize this assay for use in screening an inhibitor library and identify three novel Nrp2/VEGF-C binding inhibitors from the National Institutes of Health (NIH) Clinical Collection small molecule library.
Original language | English (US) |
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Pages (from-to) | 4-6 |
Number of pages | 3 |
Journal | Analytical biochemistry |
Volume | 453 |
Issue number | 1 |
DOIs | |
State | Published - May 15 2014 |
Externally published | Yes |
Keywords
- Assay
- Inhibitor
- Ligand
- Neuropilin
- Receptor
- VEGF
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology