MicroRNA-205 maintains T cell development following stress by regulating forkhead box N1 and selected chemokines

Ashley R. Hoover, Igor Dozmorov, Jessica MacLeod, Qiumei Du, M. De La Teresa Morena, Joseph Forbess, Kristine Guleserian, Ondine B. Cleaver, Nicolai S C Van Oers

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The thymus, an organ responsible for T cell development, is one of the more stress-sensitive tissues in the body. Stress, in the form of infections, radiation exposure, and steroids, impairs thymic epithelial cell (TEC) functions and induces the programmed cell death of immature thymocytes. MicroRNAs are small noncoding RNAs involved in tissue repair and homeostasis, with several supporting T cell development. We report that miR-205, an epithelial-specific miR, maintains thymopoiesis following inflammatory perturbations. Thus, the activation of diverse pattern recognition receptors in mice causes a more severe thymic hypoplasia and delayed T cell recovery when miR-205 is conditionally ablated in TECs. Gene expression comparisons in the TECs with/without miR-205 revealed a significant differential regulation of chemokine/chemokine receptor pathways, antigen processing components, and changes in the Wnt signaling system. This was partly a consequence of reduced expression of the transcriptional regulator of epithelial cell function, Forkhead Box N1 (Foxn1), and its two regulated targets, stem cell factor and ccl25, following stress. miR-205 mimics supplemented into miR-205-deficient fetal thymic organ cultures restored Foxn1 expression along with ccl25 and stem cell factor. A number of putative targets of miR-205 were up-regulated in TECs lacking miR-205, consistent with an important role for this miR in supporting T cell development in response to stress.

Original languageEnglish (US)
Pages (from-to)23237-23247
Number of pages11
JournalJournal of Biological Chemistry
Volume291
Issue number44
DOIs
StatePublished - Oct 28 2016

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T-cells
MicroRNAs
Chemokines
T-Lymphocytes
Stem Cell Factor
Epithelial Cells
Tissue
Pattern Recognition Receptors
Thymus
Small Untranslated RNA
Chemokine Receptors
Organ Culture Techniques
Antigen Presentation
Cell death
Thymocytes
Gene expression
Thymus Gland
Homeostasis
Repair
Cell Death

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

MicroRNA-205 maintains T cell development following stress by regulating forkhead box N1 and selected chemokines. / Hoover, Ashley R.; Dozmorov, Igor; MacLeod, Jessica; Du, Qiumei; De La Teresa Morena, M.; Forbess, Joseph; Guleserian, Kristine; Cleaver, Ondine B.; Van Oers, Nicolai S C.

In: Journal of Biological Chemistry, Vol. 291, No. 44, 28.10.2016, p. 23237-23247.

Research output: Contribution to journalArticle

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