Microsomal triglyceride transfer protein expression during mouse development

John M. Shelton, Mi Hye Lee, James A. Richardson, Shailendra B. Patel

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Feto-maternal transfer of lipophilic nutrients is an important factor in the normal development of the fetus and may be mediated by lipoproteins as carriers of these nutrients. Two proteins that may be important in this process are apolipoprotein B (apoB, the major structural protein of secreted lipoproteins) and microsomal triglyceride transfer protein (MTP) whose normal activity is required for the secretion of apoB-containing lipoproteins. Although no abnormalities of conception and embryonic lethality are known in humans who inherit genetic deficiencies of either of these proteins, homozygous mice bearing knockouts of either apoB or MTP show early embryonic lethality. To characterize the ontogeny of MTP expression during embryonic mouse development, we have used in situ hybridization to characterize the pattern of expression. By using microwave heating of tissue sections to optimize hybridization, we show that there is robust MTP expression in the yolk sac tissues followed by expression in the primordial liver cell nests as early as day 9 post-coitum (E9.5). Intestinal expression is detected around E12.5 and attains full adult expression patterns by E14.5. No expression in any other tissues was observed, including developing heart, kidney, placenta, and maternal decidua. Thus the pattern of MTP expression is compatible with a role in the transfer of lipophilic nutrients from the yolk sac, prior to hepatic development and to the liver, once the circulatory system has been established.

Original languageEnglish (US)
Pages (from-to)532-537
Number of pages6
JournalJournal of Lipid Research
Volume41
Issue number4
StatePublished - Apr 2000

Fingerprint

Apolipoproteins B
Nutrients
Lipoproteins
Yolk Sac
Tissue
Food
Liver
Bearings (structural)
Mothers
Decidua
Protein Deficiency
Proteins
Microwave heating
Microwaves
Cardiovascular System
Knockout Mice
Heating
Placenta
Embryonic Development
In Situ Hybridization

Keywords

  • Embryonic development
  • Feto-maternal unit
  • In situ hybridization
  • Microwave RNA retrieval
  • Riboprobes

ASJC Scopus subject areas

  • Endocrinology

Cite this

Microsomal triglyceride transfer protein expression during mouse development. / Shelton, John M.; Lee, Mi Hye; Richardson, James A.; Patel, Shailendra B.

In: Journal of Lipid Research, Vol. 41, No. 4, 04.2000, p. 532-537.

Research output: Contribution to journalArticle

Shelton, John M. ; Lee, Mi Hye ; Richardson, James A. ; Patel, Shailendra B. / Microsomal triglyceride transfer protein expression during mouse development. In: Journal of Lipid Research. 2000 ; Vol. 41, No. 4. pp. 532-537.
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