Midbrain dopaminergic cell loss in Parkinson's disease and MPTP-induced Parkinsonism: Sparing of calbindin-D(28k)-containing cells

D. C. German, K. F. Manaye, P. K. Sonsalla, B. A. Brooks

Research output: Contribution to journalArticle

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Abstract

Computer imaging and immunohistochemical staining techniques were used to determine which midbrain dopaminergic (DA) cells are spared in Parkinson's disease (PD), and in animals treated with the DA neurotoxin 1-methyl-4- phenyl-1,2,3,6-tetrahydropyridine (MPTP), and whether the spared cells contain the calcium-binding protein, calbindin-D(28k) (CaBP). The PD patients had more than 55% fewer midbrain DA neurons than age-matched normal subjects. The cell loss occurred within the combined substantia nigra and retrorubral area (> 61%; DA nuclei A9 and A8, respectively), and the ventral tegmental area (>42%; DA nucleus A10). The cell loss was greatest within the ventral portion of the nucleus A9. A similar pattern of DA cell loss was observed in MPTP-treated Macaca fascicularis monkeys. The CaBP-containing cells were located specifically in the cell regions spared by PD and by MPTP-treatment in both monkeys and C57BL/6 mice. These data suggest that PD and MPTP both destroy the same population of midbrain DA neurons within nuclei A8, A9, and A10, and that perhaps CaBP protects the DA neurons from cell death caused by both PD and MPTP.

Original languageEnglish (US)
Pages (from-to)42-62
Number of pages21
JournalAnnals of the New York Academy of Sciences
Volume648
StatePublished - 1992

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Calbindins
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Parkinsonian Disorders
Mesencephalon
Parkinson Disease
Neurons
Dopaminergic Neurons
antineoplaston A10
Haplorhini
Calcium-Binding Proteins
Neurotoxins
Cell death
Ventral Tegmental Area
Macaca fascicularis
Animals
Substantia Nigra
Cells
Parkinson's Disease
Inbred C57BL Mouse
Imaging techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Midbrain dopaminergic cell loss in Parkinson's disease and MPTP-induced Parkinsonism : Sparing of calbindin-D(28k)-containing cells. / German, D. C.; Manaye, K. F.; Sonsalla, P. K.; Brooks, B. A.

In: Annals of the New York Academy of Sciences, Vol. 648, 1992, p. 42-62.

Research output: Contribution to journalArticle

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