Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay

Zhi Hui Huang, Ying Wang, Li Cao, Zhi Da Su, Yan Ling Zhu, Yi Zhang Chen, Xiao Bing Yuan, Cheng He

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Olfactory ensheathing cells (OECs) are a unique type of glial cells that have axonal growth-promoting properties. OEC transplantation has emerged as a promising experimental therapy of axonal injuries and demyelinating diseases. However, some fundamental cellular properties of OECs remain unclear. In this study, we found that the distinct OEC subpopulations exhibited different migratory properties based on time-lapse imaging of single isolated cells, possibly due to their different cytoskeletal organizations. Moreover, OEC subpopulations displayed different attractive migratory responses to a gradient of lysophosphatidic acid (LPA) in single-cell migration assays. Finally, we found that OEC subpopulations transformed into each other spontaneously. Together, these results demonstrate, for the first time to our knowledge, that distinct OEC subpopulations display different migratory properties in vitro and provide new evidence to support the notion of OECs as a single cell type with malleable functional phenotypes.

Original languageEnglish (US)
Pages (from-to)479-490
Number of pages12
JournalCell Research
Volume18
Issue number4
DOIs
StatePublished - Apr 1 2008

Keywords

  • LPA
  • Morphology
  • Motility
  • Olfactory ensheathing cells
  • Single-cell migration

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Huang, Z. H., Wang, Y., Cao, L., Su, Z. D., Zhu, Y. L., Chen, Y. Z., Yuan, X. B., & He, C. (2008). Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay. Cell Research, 18(4), 479-490. https://doi.org/10.1038/cr.2008.38