Mineralocorticoid-induced membrane proteins in MDCK cells

Melanie H. Cobb, Chia Ping H Yang, Douglas M. Jefferson, Elizabeth Pasnikowski, Walter N. Scott

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-free medium. Aldosterone induced the synthesis of at least 2 membrane proteins with molecular weights of 35000 and 14000. The MDCK line may prove a useful model system for examining the mechanism of mineralocorticoid-regulated sodium transport and, in particular, the identification and study of hormone-induced proteins in a homogeneous cell population.

Original languageEnglish (US)
Pages (from-to)129-137
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume27
Issue number2
DOIs
StatePublished - 1982

Fingerprint

Mineralocorticoids
Madin Darby Canine Kidney Cells
Aldosterone
Membrane Proteins
Cells
Hormones
Desoxycorticosterone
Serum-Free Culture Media
Canidae
Proteins
Molecular Weight
Sodium
Kidney
Cell Line
Serum
Molecular weight
Population

Keywords

  • aldosterone
  • hormonally defined (serum-free) medium
  • mineralocorticoid hormones
  • sodium transport

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Mineralocorticoid-induced membrane proteins in MDCK cells. / Cobb, Melanie H.; Yang, Chia Ping H; Jefferson, Douglas M.; Pasnikowski, Elizabeth; Scott, Walter N.

In: Molecular and Cellular Endocrinology, Vol. 27, No. 2, 1982, p. 129-137.

Research output: Contribution to journalArticle

Cobb, Melanie H. ; Yang, Chia Ping H ; Jefferson, Douglas M. ; Pasnikowski, Elizabeth ; Scott, Walter N. / Mineralocorticoid-induced membrane proteins in MDCK cells. In: Molecular and Cellular Endocrinology. 1982 ; Vol. 27, No. 2. pp. 129-137.
@article{11324f8d13774b70ad5fc8d89dc3946a,
title = "Mineralocorticoid-induced membrane proteins in MDCK cells",
abstract = "The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-free medium. Aldosterone induced the synthesis of at least 2 membrane proteins with molecular weights of 35000 and 14000. The MDCK line may prove a useful model system for examining the mechanism of mineralocorticoid-regulated sodium transport and, in particular, the identification and study of hormone-induced proteins in a homogeneous cell population.",
keywords = "aldosterone, hormonally defined (serum-free) medium, mineralocorticoid hormones, sodium transport",
author = "Cobb, {Melanie H.} and Yang, {Chia Ping H} and Jefferson, {Douglas M.} and Elizabeth Pasnikowski and Scott, {Walter N.}",
year = "1982",
doi = "10.1016/0303-7207(82)90103-4",
language = "English (US)",
volume = "27",
pages = "129--137",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Mineralocorticoid-induced membrane proteins in MDCK cells

AU - Cobb, Melanie H.

AU - Yang, Chia Ping H

AU - Jefferson, Douglas M.

AU - Pasnikowski, Elizabeth

AU - Scott, Walter N.

PY - 1982

Y1 - 1982

N2 - The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-free medium. Aldosterone induced the synthesis of at least 2 membrane proteins with molecular weights of 35000 and 14000. The MDCK line may prove a useful model system for examining the mechanism of mineralocorticoid-regulated sodium transport and, in particular, the identification and study of hormone-induced proteins in a homogeneous cell population.

AB - The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-free medium. Aldosterone induced the synthesis of at least 2 membrane proteins with molecular weights of 35000 and 14000. The MDCK line may prove a useful model system for examining the mechanism of mineralocorticoid-regulated sodium transport and, in particular, the identification and study of hormone-induced proteins in a homogeneous cell population.

KW - aldosterone

KW - hormonally defined (serum-free) medium

KW - mineralocorticoid hormones

KW - sodium transport

UR - http://www.scopus.com/inward/record.url?scp=0019992125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019992125&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(82)90103-4

DO - 10.1016/0303-7207(82)90103-4

M3 - Article

C2 - 7117689

AN - SCOPUS:0019992125

VL - 27

SP - 129

EP - 137

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 2

ER -