Mineralocorticoid-induced membrane proteins in MDCK cells

Melanie H. Cobb; Chia Ping H Yang; Douglas M. Jefferson; Elizabeth Pasnikowski; Walter N. Scott

doi:10.1016/0303-7207(82)90103-4

Mineralocorticoid-induced membrane proteins in MDCK cells

Melanie H. Cobb, Chia Ping H Yang, Douglas M. Jefferson, Elizabeth Pasnikowski, Walter N. Scott

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-free medium. Aldosterone induced the synthesis of at least 2 membrane proteins with molecular weights of 35000 and 14000. The MDCK line may prove a useful model system for examining the mechanism of mineralocorticoid-regulated sodium transport and, in particular, the identification and study of hormone-induced proteins in a homogeneous cell population.

Original language	English (US)
Pages (from-to)	129-137
Number of pages	9
Journal	Molecular and Cellular Endocrinology
Volume	27
Issue number	2
DOIs	https://doi.org/10.1016/0303-7207(82)90103-4
State	Published - Jul 1982

Keywords

aldosterone
hormonally defined (serum-free) medium
mineralocorticoid hormones
sodium transport

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

Access to Document

10.1016/0303-7207(82)90103-4

Cite this

@article{11324f8d13774b70ad5fc8d89dc3946a,

title = "Mineralocorticoid-induced membrane proteins in MDCK cells",

abstract = "The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-free medium. Aldosterone induced the synthesis of at least 2 membrane proteins with molecular weights of 35000 and 14000. The MDCK line may prove a useful model system for examining the mechanism of mineralocorticoid-regulated sodium transport and, in particular, the identification and study of hormone-induced proteins in a homogeneous cell population.",

keywords = "aldosterone, hormonally defined (serum-free) medium, mineralocorticoid hormones, sodium transport",

author = "Cobb, {Melanie H.} and Yang, {Chia Ping H} and Jefferson, {Douglas M.} and Elizabeth Pasnikowski and Scott, {Walter N.}",

year = "1982",

month = jul,

doi = "10.1016/0303-7207(82)90103-4",

language = "English (US)",

volume = "27",

pages = "129--137",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Mineralocorticoid-induced membrane proteins in MDCK cells

AU - Cobb, Melanie H.

AU - Yang, Chia Ping H

AU - Jefferson, Douglas M.

AU - Pasnikowski, Elizabeth

AU - Scott, Walter N.

PY - 1982/7

Y1 - 1982/7

N2 - The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-free medium. Aldosterone induced the synthesis of at least 2 membrane proteins with molecular weights of 35000 and 14000. The MDCK line may prove a useful model system for examining the mechanism of mineralocorticoid-regulated sodium transport and, in particular, the identification and study of hormone-induced proteins in a homogeneous cell population.

AB - The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-free medium. Aldosterone induced the synthesis of at least 2 membrane proteins with molecular weights of 35000 and 14000. The MDCK line may prove a useful model system for examining the mechanism of mineralocorticoid-regulated sodium transport and, in particular, the identification and study of hormone-induced proteins in a homogeneous cell population.

KW - aldosterone

KW - hormonally defined (serum-free) medium

KW - mineralocorticoid hormones

KW - sodium transport

UR - http://www.scopus.com/inward/record.url?scp=0019992125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019992125&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(82)90103-4

DO - 10.1016/0303-7207(82)90103-4

M3 - Article

C2 - 7117689

AN - SCOPUS:0019992125

SN - 0303-7207

VL - 27

SP - 129

EP - 137

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 2

ER -

Mineralocorticoid-induced membrane proteins in MDCK cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this