MIP-1γ: Molecular cloning, expression, and biological activities of a novel CC chemokine that is constitutively secreted in vivo

A. N. Poltorak, F. Bazzoni, I. I. Smirnova, E. Alejos, P. Thompson, G. Luheshi, N. Rothwell, B. Beutler

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

We have identified a novel CC chemokine family member, herein termed MIP- 1γ in view of its similarity to existing members of the MIP-1 group. The murine protein has a predicted length of 100 amino acids. Like MIP-1α, recombinant MIP-1γ acts as a pyrogen when administered intracerebroventricularly. MIP-1γ, and MIP-1α engage the same high-affinity receptor on neutrophils, activating calcium release within seconds following cell contact. Pretreatment with either chemokine abolishes responses to the other, and to itself, suggesting utilization of a common signaling pathway. However, unlike MIP-1α or any of the other CC chemokines, MIP-1γ is expressed constitutively by a wide variety of tissues, and circulates in the blood of healthy mice at concentrations of approximately 1 μg/ml (90 nM). It would therefore be predicted that MIP-1γ occupies most of the CC chemokine receptors that exist in the intravascular compartment. As such it might, under normal circumstances, markedly influence responses to the inducible CC chemokines.

Original languageEnglish (US)
Pages (from-to)207-219
Number of pages13
JournalJournal of Inflammation
Volume45
Issue number3
StatePublished - Jan 1 1995

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Keywords

  • CC chemokine
  • MIP-1α
  • MIP-1γ
  • murine protein
  • neutrophils

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Poltorak, A. N., Bazzoni, F., Smirnova, I. I., Alejos, E., Thompson, P., Luheshi, G., Rothwell, N., & Beutler, B. (1995). MIP-1γ: Molecular cloning, expression, and biological activities of a novel CC chemokine that is constitutively secreted in vivo. Journal of Inflammation, 45(3), 207-219.