miR-200 regulates endometrial development during early pregnancy

Patricia T. Jimenez, Monica A. Mainigi, R. Ann Word, W. Le Kraus, Carole R. Mendelson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

For successful embryo implantation, endometrial stromal cells must undergo functional and morphological changes, referred to as decidualization. However, the molecular mechanisms that regulate implantation and decidualization are not well defined. Here we demonstrate that the estradiol- and progesterone-regulated microRNA (miR)-200 family was markedly down-regulated in mouse endometrial stromal cells prior to implantation, whereas zinc finger E-box binding homeobox-1 and -2 and other known and predicted targets were up-regulated. Conversely, miR-200 was up-regulated during in vitro decidualization of human endometrial stromal cells. Knockdown of miR-200 negatively affected decidualization and prevented the mesenchymal-epithelial transition-like changes that accompanied decidual differentiation. Notably, superovulation of mice and humans altered miR-200 expression. Our findings suggest that hormonal alterations that accompany superovulation may negatively impact endometrial development and decidualization by causing aberrant miR-200 expression.

Original languageEnglish (US)
Pages (from-to)977-987
Number of pages11
JournalMolecular Endocrinology
Volume30
Issue number9
DOIs
StatePublished - Sep 1 2016

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MicroRNAs
Pregnancy
Stromal Cells
Superovulation
Epithelial-Mesenchymal Transition
Progesterone
Estradiol

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

miR-200 regulates endometrial development during early pregnancy. / Jimenez, Patricia T.; Mainigi, Monica A.; Word, R. Ann; Kraus, W. Le; Mendelson, Carole R.

In: Molecular Endocrinology, Vol. 30, No. 9, 01.09.2016, p. 977-987.

Research output: Contribution to journalArticle

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