miR-206 and -486 induce myoblast differentiation by downregulating Pax7

Bijan K. Dey, Jeffrey Gagan, Anindya Dutta

Research output: Contribution to journalArticle

250 Citations (Scopus)

Abstract

The Pax7 transcription factor is required for muscle satellite cell biogenesis and specification of the myogenic precursor lineage. Pax7 is expressed in proliferating myoblasts but is rapidly downregulated during differentiation. Here we report that miR-206 and -486 are induced during myoblast differentiation and downregulate Pax7 by directly targeting its 3′ untranslated region (UTR). Expression of either of these microRNAs in myoblasts accelerates differentiation, whereas inhibition of these microRNAs causes persistence of Pax7 protein and delays differentiation. A microRNA-resistant form of Pax7 is sufficient to inhibit differentiation. Since both these microRNAs are induced by MyoD and since Pax7 promotes the expression of Id2, an inhibitor of MyoD, our results revealed a bistable switch that exists either in a Pax7-driven myoblast state or a MyoD-driven myotube state.

Original languageEnglish (US)
Pages (from-to)203-214
Number of pages12
JournalMolecular and cellular biology
Volume31
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

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Myoblasts
MicroRNAs
Down-Regulation
Skeletal Muscle Fibers
3' Untranslated Regions
Muscle Cells
Transcription Factors
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

miR-206 and -486 induce myoblast differentiation by downregulating Pax7. / Dey, Bijan K.; Gagan, Jeffrey; Dutta, Anindya.

In: Molecular and cellular biology, Vol. 31, No. 1, 01.01.2011, p. 203-214.

Research output: Contribution to journalArticle

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