MiR-277/4989 regulate transcriptional landscape during juvenile to adult transition in the parasitic helminth Schistosoma mansoni

Anna V. Protasio, Stijn van Dongen, Julie Collins, Leonor Quintais, Diogo M. Ribeiro, Florian Sessler, Martin Hunt, Gabriel Rinaldi, James J. Collins, Anton J. Enright, Matthew Berriman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Schistosomes are parasitic helminths that cause schistosomiasis, a disease affecting circa 200 million people, primarily in underprivileged regions of the world. Schistosoma mansoni is the most experimentally tractable schistosome species due to its ease of propagation in the laboratory and the high quality of its genome assembly and annotation. Although there is growing interest in microRNAs (miRNAs) in trematodes, little is known about the role these molecules play in the context of developmental processes. We use the completely unaware “miRNA-blind” bioinformatics tool Sylamer to analyse the 3’-UTRs of transcripts differentially expressed between the juvenile and adult stages. We show that the miR-277/4989 family target sequence is the only one significantly enriched in the transition from juvenile to adult worms. Further, we describe a novel miRNA, sma-miR-4989 showing that its proximal genomic location to sma-miR-277 suggests that they form a miRNA cluster, and we propose hairpin folds for both miRNAs compatible with the miRNA pathway. In addition, we found that expression of sma-miR-277/4989 miRNAs are up-regulated in adults while their predicted targets are characterised by significant down-regulation in paired adult worms but remain largely undisturbed in immature “virgin” females. Finally, we show that sma-miR-4989 is expressed in tegumental cells located proximal to the oesophagus gland and also distributed throughout the male worms’ body. Our results indicate that sma-miR-277/4989 might play a dominant role in post-transcriptional regulation during development of juvenile worms and suggest an important role in the sexual development of female schistosomes.

Original languageEnglish (US)
Article numbere0005559
JournalPLoS Neglected Tropical Diseases
Volume11
Issue number5
DOIs
StatePublished - May 23 2017

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Schistosoma mansoni
Helminths
MicroRNAs
Sexual Development
Schistosomiasis
3' Untranslated Regions
Computational Biology
Esophagus
Down-Regulation
Genome

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Protasio, A. V., van Dongen, S., Collins, J., Quintais, L., Ribeiro, D. M., Sessler, F., ... Berriman, M. (2017). MiR-277/4989 regulate transcriptional landscape during juvenile to adult transition in the parasitic helminth Schistosoma mansoni. PLoS Neglected Tropical Diseases, 11(5), [e0005559]. https://doi.org/10.1371/journal.pntd.0005559

MiR-277/4989 regulate transcriptional landscape during juvenile to adult transition in the parasitic helminth Schistosoma mansoni. / Protasio, Anna V.; van Dongen, Stijn; Collins, Julie; Quintais, Leonor; Ribeiro, Diogo M.; Sessler, Florian; Hunt, Martin; Rinaldi, Gabriel; Collins, James J.; Enright, Anton J.; Berriman, Matthew.

In: PLoS Neglected Tropical Diseases, Vol. 11, No. 5, e0005559, 23.05.2017.

Research output: Contribution to journalArticle

Protasio, AV, van Dongen, S, Collins, J, Quintais, L, Ribeiro, DM, Sessler, F, Hunt, M, Rinaldi, G, Collins, JJ, Enright, AJ & Berriman, M 2017, 'MiR-277/4989 regulate transcriptional landscape during juvenile to adult transition in the parasitic helminth Schistosoma mansoni', PLoS Neglected Tropical Diseases, vol. 11, no. 5, e0005559. https://doi.org/10.1371/journal.pntd.0005559
Protasio, Anna V. ; van Dongen, Stijn ; Collins, Julie ; Quintais, Leonor ; Ribeiro, Diogo M. ; Sessler, Florian ; Hunt, Martin ; Rinaldi, Gabriel ; Collins, James J. ; Enright, Anton J. ; Berriman, Matthew. / MiR-277/4989 regulate transcriptional landscape during juvenile to adult transition in the parasitic helminth Schistosoma mansoni. In: PLoS Neglected Tropical Diseases. 2017 ; Vol. 11, No. 5.
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