MiR-335 and miR-363 regulation of neuroblastoma tumorigenesis and metastasis

Jingbo Qiao, Sora Lee, Pritha Paul, Lauren Theiss, Joshua Tiao, Lan Qiao, Andrew Kong, Dai H. Chung

Research output: Contribution to journalArticle

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Abstract

Background: microRNA (miRNA) functions broadly as post-transcriptional regulators of gene expression, and disproportionate miRNAs can result in dysregulation of oncogenes in cancer cells. We have previously shown that gastrin-releasing peptide receptor (GRP-R) signaling regulates tumorigenicity of neuroblastoma cells. Herein, we sought to characterize miRNA profile in GRP-R silenced neuroblastoma cells, and to determine the role of miRNAs on tumorigenicity and metastatic potential. Methods: Human neuroblastoma cell lines, BE(2)-C and SK-N-SH, were used for our study. Stably transfected GRP-R silenced cells were assessed for miRNA profiles. Cells were transfected with miR-335, miR-363, or miR-CON, a nontargeting control, and in vitro assays were performed. In vivo functions of miR-335 and miR-363 were also assessed in a spleen-liver metastasis murine model. Results: GRP-R silencing significantly increased expression of miR-335 and miR-363 in BE(2)-C cells. Overexpression of miR-335 and miR-363 decreased tumorigenicity as measured by clonogenicity, anchorage-independent growth, and metastasis determined by cell invasion assay and liver metastasis in vivo. Conclusion: We report, for the first time, that GRP-R-mediated tumorigenicity and increased metastatic potential in neuroblastoma are regulated, in part, by miR-335 and miR-363. A better understanding of the anti-tumor functions of miRNAs could provide valuable insights to discerning molecular mechanisms responsible for neuroblastoma metastasis.

Original languageEnglish (US)
Pages (from-to)226-233
Number of pages8
JournalSurgery (United States)
Volume154
Issue number2
DOIs
StatePublished - Aug 1 2013
Externally publishedYes

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Bombesin Receptors
Neuroblastoma
MicroRNAs
Carcinogenesis
Neoplasm Metastasis
Liver
Regulator Genes
Oncogenes
Neoplasms
Spleen
Gene Expression
Cell Line
Growth

ASJC Scopus subject areas

  • Surgery

Cite this

MiR-335 and miR-363 regulation of neuroblastoma tumorigenesis and metastasis. / Qiao, Jingbo; Lee, Sora; Paul, Pritha; Theiss, Lauren; Tiao, Joshua; Qiao, Lan; Kong, Andrew; Chung, Dai H.

In: Surgery (United States), Vol. 154, No. 2, 01.08.2013, p. 226-233.

Research output: Contribution to journalArticle

Qiao, Jingbo ; Lee, Sora ; Paul, Pritha ; Theiss, Lauren ; Tiao, Joshua ; Qiao, Lan ; Kong, Andrew ; Chung, Dai H. / MiR-335 and miR-363 regulation of neuroblastoma tumorigenesis and metastasis. In: Surgery (United States). 2013 ; Vol. 154, No. 2. pp. 226-233.
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