@article{efacb920a1ae44e595de7b15a600b0ae,
title = "MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary",
abstract = "MicroRNAs (miRNAs) are processed from primary miRNA transcripts (pri-miRNAs), many of which are annotated as long noncoding RNAs (lncRNAs). We assessed whether MIR205HG, the host gene for miR-205, has independent functions as an lncRNA. Comparing mice with targeted deletions of MIR205HG and miR-205 revealed a functional role for the lncRNA in the anterior pituitary. Mice lacking MIR205HG had a temporal reduction in Pit1, growth hormone, and prolactin. This was mediated, in part, through the ability of this lncRNA to bind and regulate the transcriptional activity of Pit1 in conjunction with Zbtb20. Knockdown of MIR205HG in lactotropes decreased the expression of Pit1, Zbtb20, prolactin, and growth hormone, while its overexpression enhanced the levels of these transcripts. The effects of MIR205HG on the pituitary were independent of miR-205. The data support a role for MIR205HG as an lncRNA that regulates growth hormone and prolactin production in the anterior pituitary. MIR205HG is an lncRNA that harbors the gene for miR-205, an miRNA expressed in epithelial cells. Du et al. show that mouse MIR205HG functions in the anterior pituitary, independently of miR-205, to regulate growth. The lncRNA acts with the transcription factor Pit1 to regulate expression of growth hormone and prolactin.",
keywords = "MIR205HG, endocrine hormones, growth hormone, miR-205, pituitary gland, pituitary transcriptome, pouf1, prolactin, thymus",
author = "Qiumei Du and Hoover, {Ashley R.} and Igor Dozmorov and Prithvi Raj and Shaheen Khan and Erika Molina and Chang, {Tsung Cheng} and {de la Morena}, {Maria Teresa} and Cleaver, {Ondine B.} and Mendell, {Joshua T.} and {van Oers}, {Nicolai S.C.}",
note = "Funding Information: We would like to thank Angela Mobley and Ian Mathews from the flow cytometry core for assistance in TEC sorting and flow analyses. We appreciate the input from Ms. Fatma Coskun (van Oers laboratory) in RNA-protein studies. We are grateful for the help provided by Drs. Leilani Marty-Santos and Caitlyn Braisch in Dr. Ondine Cleaver's laboratory. We are very thankful for help with RNA-protein interaction studies from Ms. Anu Thomas (Mendell laboratory) and Dr. Nicholas Conrad and Mr. Ian Boys (Department of Microbiology, UT Southwestern Medical Center). We thank Drs. Rhonda Bassel-Duby and Eric Olson for helpful discussions and Mr. Jose Cabrera for image preparations (Department of Molecular Biology at UT Southwestern Medical Center). Dr. James Richardson, John Shelton, and staff provided help with the processing of pituitary tissue and lacrimal glands and are part of the histology core. The MIR205HG-targeted mice and miR-205 knockin mice were generated with the help of Dr. Robert Hammer and staff from the Transgenic and Knockout Core at UT Southwestern Medical Center. The primary antibodies used to identify pituitary cell lineages were provided by Dr. A.F. Parlow from the Harbor-UCLA Research and Education Institute. The ΔNp63 construct was graciously sent by Dr. S. Sinha (University at Buffalo, NY). This work was supported, in part, by grants from the National Institutes of Health R01, United States (AI114523, N.S.C.v.O.), F31 (AI110140, A.R.H.), as well as Children's Medical Center Research Bridge Support, United States (N.S.C.v.O. and M.T.d.l.M.), Beecherl funds from the Department of Immunology at UT Southwestern Medical Center, United States (N.S.C.v.O.), and the Jeffrey Modell Foundation, United States (M.T.d.l.M.). Q.D. A.R.H. M.T.d.l.M. and N.S.C.v.O. conceived the experiments. Q.D. A.R.H. I.D. P.R. S.K. E.M. and N.S.C.v.O. performed the experiments. I.D. P.R. and S.K. analyzed the RNA-seq and array data. Q.D. A.R.H. I.D. T.-C.C. M.T.d.l.M. O.B.C. J.T.M. and N.S.C.v.O. analyzed the data. Q.D. and N.S.C.v.O. wrote the manuscript. J.T.M. reviewed the manuscript. J.T.M. is on the scientific advisory board of a company called Ribometrix, which makes small molecules that target RNAs. There is no direct relationship between his role with the company and the work described in the current manuscript. Funding Information: We would like to thank Angela Mobley and Ian Mathews from the flow cytometry core for assistance in TEC sorting and flow analyses. We appreciate the input from Ms. Fatma Coskun (van Oers laboratory) in RNA-protein studies. We are grateful for the help provided by Drs. Leilani Marty-Santos and Caitlyn Braisch in Dr. Ondine Cleaver{\textquoteright}s laboratory. We are very thankful for help with RNA-protein interaction studies from Ms. Anu Thomas (Mendell laboratory) and Dr. Nicholas Conrad and Mr. Ian Boys (Department of Microbiology, UT Southwestern Medical Center). We thank Drs. Rhonda Bassel-Duby and Eric Olson for helpful discussions and Mr. Jose Cabrera for image preparations (Department of Molecular Biology at UT Southwestern Medical Center). Dr. James Richardson, John Shelton, and staff provided help with the processing of pituitary tissue and lacrimal glands and are part of the histology core. The MIR205HG -targeted mice and miR-205 knockin mice were generated with the help of Dr. Robert Hammer and staff from the Transgenic and Knockout Core at UT Southwestern Medical Center. The primary antibodies used to identify pituitary cell lineages were provided by Dr. A.F. Parlow from the Harbor-UCLA Research and Education Institute. The ΔNp63 construct was graciously sent by Dr. S. Sinha (University at Buffalo, NY). This work was supported, in part, by grants from the National Institutes of Health R01, United States ( AI114523 , N.S.C.v.O.), F31 ( AI110140 , A.R.H.), as well as Children{\textquoteright}s Medical Center Research Bridge Support, United States (N.S.C.v.O. and M.T.d.l.M.), Beecherl funds from the Department of Immunology at UT Southwestern Medical Center , United States (N.S.C.v.O.), and the Jeffrey Modell Foundation , United States (M.T.d.l.M.). Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = may,
day = "20",
doi = "10.1016/j.devcel.2019.03.012",
language = "English (US)",
volume = "49",
pages = "618--631.e5",
journal = "Developmental cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "4",
}