Mitochondrial transformation of mammalian cells

Mike A. Clark, Jerry W. Shay

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Resistance to the antibiotics chloramphenicol (CAPr) and efrapeptin (EFr) in mammalian cells is cytoplasmically inherited 1,2. We demonstrate here that purified mitochondria obtained from CAPr and EFr cells are taken up by endocytosis and transfer antibiotic resistance to chloramphenicol-(CAPs) and efrapeptin-(EFs) sensitive cells. The resultant cells, termed mitochondrial transformants, are stably resistant to the antibiotics and are produced with high efficiency. The ability to transfer antibiotic resistance using mitochondria supports the notion that CAPr is due to a mutation of the mitochondrial DNA3. This technique of mitochondrial-mediated transfer of antibiotic resistance may provide a novel means for studying mitochondrial genetics in mammalian cells.

Original languageEnglish (US)
Pages (from-to)605-607
Number of pages3
JournalNature
Volume295
Issue number5850
DOIs
Publication statusPublished - 1982

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