Mitogenic activation of B cells in vitro: The properties of adherent accessory cells as revealed by partition analysis

J. R. Kettman, A. Soederberg, I. Lefkovits

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The requirement of B cells activated by mitogen (dextran sulfate plus lipopolysaccharide) for accessory cells was studied by partition analysis. Small numbers of splenic B cells were activated to clonal growth, as determined by visual inspection, and to immunoglobulin (Ig) synthesis, as determined by release of Ig into the culture fluid. By placing irradiated adherent cells in the periphery of the micro-culture wells and forcing responding cells to different areas of the well (slant experiments), it was observed that no cell contact was necessary for B cell activation, and that 'promoted' contact ('Rock and Roll' experiments) does not increase the efficiency of activation. Sequential microcultures suggest that only some irradiated adherent cells act as accessory cells, but they can perform this function to more than one B cell. Attempts to perform limiting dilution analysis by varying irradiated adherent cell input showed non-single-hit behavior. When the data were rearranged, taking into account the distribution of irradiated adherent cells, then single-hit behavior with about 1 to 5% of irradiated adherent cells acting as an accessory cells for B cell clonal activation was observed. The evidence suggests that an uncommon irradiated adherent cell releases a soluble factor necessary for B cell activation and/or clonal proliferation.

Original languageEnglish (US)
Pages (from-to)1144-1148
Number of pages5
JournalJournal of Immunology
Volume137
Issue number4
StatePublished - 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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