Mitogenic repression of myogenin autoregulation

Diane G. Edmondson, Thomas J. Brennan, Eric N. Olson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The muscle-specific helix-loop-helix proteins MyoD and myogenin have been shown to positively autoregulate their own expression and to cross-activate one another's expression following transfection into a variety of nonmyogenic cell lines. We show that the ability of an exogenous myogenin expression vector to activate the endogenous myogenin allele in 10T1/2 fibroblasts is dependent on serum withdrawal. Repression of myogenin autoregulation by serum can be observed with a reporter gene linked to myogenin upstream sequences, indicating that repression occurs at the level of transcription. Like MyoD, myogenin exhibits a short half-life (∼20 min), which may serve to maintain the autoregulatory loop without allowing excess accumulation of the protein.

Original languageEnglish (US)
Pages (from-to)21343-21346
Number of pages4
JournalJournal of Biological Chemistry
Volume266
Issue number32
StatePublished - 1991

Fingerprint

Myogenin
Homeostasis
MyoD Protein
Transcription
Fibroblasts
Serum
Reporter Genes
Transfection
Half-Life
Muscle
Genes
Alleles
Cells
Cell Line
Muscles

ASJC Scopus subject areas

  • Biochemistry

Cite this

Edmondson, D. G., Brennan, T. J., & Olson, E. N. (1991). Mitogenic repression of myogenin autoregulation. Journal of Biological Chemistry, 266(32), 21343-21346.

Mitogenic repression of myogenin autoregulation. / Edmondson, Diane G.; Brennan, Thomas J.; Olson, Eric N.

In: Journal of Biological Chemistry, Vol. 266, No. 32, 1991, p. 21343-21346.

Research output: Contribution to journalArticle

Edmondson, DG, Brennan, TJ & Olson, EN 1991, 'Mitogenic repression of myogenin autoregulation', Journal of Biological Chemistry, vol. 266, no. 32, pp. 21343-21346.
Edmondson, Diane G. ; Brennan, Thomas J. ; Olson, Eric N. / Mitogenic repression of myogenin autoregulation. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 32. pp. 21343-21346.
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