Mitogenic repression of myogenin autoregulation

Diane G. Edmondson, Thomas J. Brennan, Eric N. Olson

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

The muscle-specific helix-loop-helix proteins MyoD and myogenin have been shown to positively autoregulate their own expression and to cross-activate one another's expression following transfection into a variety of nonmyogenic cell lines. We show that the ability of an exogenous myogenin expression vector to activate the endogenous myogenin allele in 10T1/2 fibroblasts is dependent on serum withdrawal. Repression of myogenin autoregulation by serum can be observed with a reporter gene linked to myogenin upstream sequences, indicating that repression occurs at the level of transcription. Like MyoD, myogenin exhibits a short half-life (~20 min), which may serve to maintain the autoregulatory loop without allowing excess accumulation of the protein.

Original languageEnglish (US)
Pages (from-to)21343-21346
Number of pages4
JournalJournal of Biological Chemistry
Volume266
Issue number32
StatePublished - 1991

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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