Mitomycin C resistance in a human colon carcinoma cell line associated with cell surface protein alterations

J. K. Willson, B. H. Long, M. E. Marks, D. E. Brattain, J. E. Wiley, M. G. Brattain

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Abstract

A human colon carcinoma cell line resistant to mitomycin C (MMC) was obtained by repeated exposure to a previously described sensitive parental line, HCT 116, to MMC in vitro. Xenografts grown from the MMC-resistant phenotype were not inhibited in MMC-treated animals, while MMC treatment produced growth inhibition in parental cell xenografts. The MMC-resistant phenotype exhibited a greater amount of a M(r) 148,000 cell surface protein than did the parental line. The increase in this M(r) 148,000 cell surface protein correlated positively with the degree of MMC resistance. Alkaline elution of filter-bound DNA from resistant cells exposed to MMC in vitro showed a decrease in DNA cross-link formation such that a 10-fold higher MMC concentration was required to produce similar cross-link formation in the resistant cell as compared to the parental cell. The development of MMC resistance was not associated with in vitro cross-resistance to other natural product cytotoxic drugs. This model for resistance to MMC will be useful in future studies to define the mechanisms for MMC action and resistance in human colon carcinoma cells.

Original languageEnglish (US)
Pages (from-to)5880-5885
Number of pages6
JournalCancer Research
Volume44
Issue number12 I
StatePublished - 1984

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Mitomycin
Membrane Proteins
Colon
Carcinoma
Cell Line
Heterografts
Phenotype
DNA
Biological Products

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Willson, J. K., Long, B. H., Marks, M. E., Brattain, D. E., Wiley, J. E., & Brattain, M. G. (1984). Mitomycin C resistance in a human colon carcinoma cell line associated with cell surface protein alterations. Cancer Research, 44(12 I), 5880-5885.

Mitomycin C resistance in a human colon carcinoma cell line associated with cell surface protein alterations. / Willson, J. K.; Long, B. H.; Marks, M. E.; Brattain, D. E.; Wiley, J. E.; Brattain, M. G.

In: Cancer Research, Vol. 44, No. 12 I, 1984, p. 5880-5885.

Research output: Contribution to journalArticle

Willson, JK, Long, BH, Marks, ME, Brattain, DE, Wiley, JE & Brattain, MG 1984, 'Mitomycin C resistance in a human colon carcinoma cell line associated with cell surface protein alterations', Cancer Research, vol. 44, no. 12 I, pp. 5880-5885.
Willson JK, Long BH, Marks ME, Brattain DE, Wiley JE, Brattain MG. Mitomycin C resistance in a human colon carcinoma cell line associated with cell surface protein alterations. Cancer Research. 1984;44(12 I):5880-5885.
Willson, J. K. ; Long, B. H. ; Marks, M. E. ; Brattain, D. E. ; Wiley, J. E. ; Brattain, M. G. / Mitomycin C resistance in a human colon carcinoma cell line associated with cell surface protein alterations. In: Cancer Research. 1984 ; Vol. 44, No. 12 I. pp. 5880-5885.
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