MitoNEET-mediated effects on browning of white adipose tissue

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

MitoNEET is an outer mitochondrial membrane protein that, upon overexpression in white adipose tissue (WAT), exerts a positive impact on tissue expansion and whole-body lipid and carbohydrate homeostasis by altering mitochondrial matrix iron metabolism. Here we determine the key transcriptional events in subcutaneous WAT of mice in response to mitoNEET overexpression and a high-fat diet (HFD). Microarray analyses at key points during weight gain upon body weight divergence with wild-type mice demonstrate that mitoNEET-enriched sWAT early on, upregulates a browning signature programme that limits WAT expansion in transgenic mice for a period of up to 12 weeks of HFD. This compensatory browning phenotype is subsequently lost, resulting in rapid WAT expansion and body weight gain. Exposure to thermoneutral temperatures during HFD prompts weight gain significantly earlier. Similar WAT expansion is achieved upon infection with an adeno-associated virus expressing mitoNEET. Collectively, the mitoNEET-enriched fat pads feature a more vascularized, anti-inflammatory and less fibrotic environment.

Original languageEnglish (US)
Article number3962
JournalNature Communications
Volume5
DOIs
StatePublished - May 28 2014

Fingerprint

adipose tissues
White Adipose Tissue
Tissue Expansion
fats
diets
Tissue
High Fat Diet
Weight Gain
mice
Nutrition
body weight
Fats
expansion
Body Weight
homeostasis
Dependovirus
phenotype
Subcutaneous Fat
Mitochondrial Proteins
carbohydrates

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

MitoNEET-mediated effects on browning of white adipose tissue. / Kusminski, Christine M.; Park, Jiyoung; Scherer, Philipp E.

In: Nature Communications, Vol. 5, 3962, 28.05.2014.

Research output: Contribution to journalArticle

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