Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells

Jungseog Kang, Jaideep Chaudhary, Hui Dong, Soonjoung Kim, Chad A. Brautigam, Hongtao Yua

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Human Shugoshin 1 (Sgo1) protects centromeric sister-chromatid cohesion during prophase and prevents premature sister-chromatid separation. Heterochromatin protein 1 (HP1) has been proposed to protect centromeric sister-chromatid cohesion by directly targeting Sgo1 to centromeres in mitosis. Here we show that HP1a is targeted to mitotic centromeres by INCENP, a subunit of the chromosome passenger complex (CPC). Biochemical and structural studies show that both HP1-INCENP and HP1-Sgo1 interactions require the binding of the HP1 chromo shadow domain to PXVXL/I motifs in INCENP or Sgo1, suggesting that the INCENP-bound, centromeric HP1a is incapable of recruiting Sgo1. Consistently, a Sgo1 mutant deficient in HP1 binding is functional in centromeric cohesion protection and localizes normally to centromeres in mitosis. By contrast, INCENP or Sgo1 mutants deficient in HP1 binding fail to localize to centromeres in interphase. Therefore, our results suggest that HP1 binding by INCENP or Sgo1 is dispensable for centromeric cohesion protection during mitosis of human cells, but might regulate yet uncharacterized interphase functions of CPC or Sgo1 at the centromeres.

Original languageEnglish (US)
Pages (from-to)1181-1190
Number of pages10
JournalMolecular Biology of the Cell
Volume22
Issue number8
DOIs
StatePublished - Apr 15 2011

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Chromatids
Centromere
Mitosis
Protein Binding
Interphase
Chromosomes
Prophase
heterochromatin-specific nonhistone chromosomal protein HP-1
Carrier Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells. / Kang, Jungseog; Chaudhary, Jaideep; Dong, Hui; Kim, Soonjoung; Brautigam, Chad A.; Yua, Hongtao.

In: Molecular Biology of the Cell, Vol. 22, No. 8, 15.04.2011, p. 1181-1190.

Research output: Contribution to journalArticle

Kang, Jungseog ; Chaudhary, Jaideep ; Dong, Hui ; Kim, Soonjoung ; Brautigam, Chad A. ; Yua, Hongtao. / Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 8. pp. 1181-1190.
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