Mitotic cytosol inhibits invagination of coated pits in broken mitotic cells

Marc Pypaert, Dorothy Mundy, Ewen Souter, Jean Claude Labbé, Graham Warren

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Receptor-mediated endocytosis is inhibited during mitosis in mammalian cells and earlier work on A431 cells suggested that one of the sites inhibited was the invagination of coated pits (Pypaert, M., J. M. Lucocq, and G. Warren. 1987. Eur. J. Cell Biol. 45: 23-29). To explore this inhibition further, we have reproduced it in broken HeLa cells. Mitotic or interphase cells were broken by freeze-thawing in liquid nitrogen and warmed in the presence of mitotic or interphase cytosol. Using a morphological assay, we found invagination to be inhibited only when mitotic cells were incubated in mitotic cytosol. This inhibition was reversed by diluting the cytosol during the incubation. Reversal was sensitive to okadaic acid, a potent phosphatase inhibitor, showing that phosphorylation was involved in the inhibition of invagination. This was confirmed using purified cdc2 kinase which alone could partially substitute for mitotic cytosol.

Original languageEnglish (US)
Pages (from-to)1159-1166
Number of pages8
JournalJournal of Cell Biology
Volume114
Issue number6
StatePublished - Sep 1991

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Cytosol
Interphase
Okadaic Acid
Endocytosis
HeLa Cells
Phosphoric Monoester Hydrolases
Mitosis
Phosphotransferases
Nitrogen
Phosphorylation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Pypaert, M., Mundy, D., Souter, E., Labbé, J. C., & Warren, G. (1991). Mitotic cytosol inhibits invagination of coated pits in broken mitotic cells. Journal of Cell Biology, 114(6), 1159-1166.

Mitotic cytosol inhibits invagination of coated pits in broken mitotic cells. / Pypaert, Marc; Mundy, Dorothy; Souter, Ewen; Labbé, Jean Claude; Warren, Graham.

In: Journal of Cell Biology, Vol. 114, No. 6, 09.1991, p. 1159-1166.

Research output: Contribution to journalArticle

Pypaert, M, Mundy, D, Souter, E, Labbé, JC & Warren, G 1991, 'Mitotic cytosol inhibits invagination of coated pits in broken mitotic cells', Journal of Cell Biology, vol. 114, no. 6, pp. 1159-1166.
Pypaert M, Mundy D, Souter E, Labbé JC, Warren G. Mitotic cytosol inhibits invagination of coated pits in broken mitotic cells. Journal of Cell Biology. 1991 Sep;114(6):1159-1166.
Pypaert, Marc ; Mundy, Dorothy ; Souter, Ewen ; Labbé, Jean Claude ; Warren, Graham. / Mitotic cytosol inhibits invagination of coated pits in broken mitotic cells. In: Journal of Cell Biology. 1991 ; Vol. 114, No. 6. pp. 1159-1166.
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