Mitotic phosphorylation of histone H3 is governed by IpI1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes

Jer Yuan Hsu, Zu Wen Sun, Xiumin Li, Melanie Reuben, Kelly Tatchell, Douglas K. Bishop, Jeremy M. Grushcow, Cynthia J. Brame, Jennifer A. Caldwell, Donald F. Hunt, Rueyling Lin, M. Mitchell Smith, C. David Allis

Research output: Contribution to journalArticle

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Abstract

Phosphorylation of histone H3 at serine 10 occurs during mitosis and meiosis in a wide range of eukaryotes and has been shown to be required for proper chromosome transmission in Tetrahymena. Here we report that IpI1/aurora kinase and its genetically interacting phosphatase, GIc7/PP1, are responsible for the balance of H3 phosphorylation during mitosis in Saccharomyces cerevisiae and Caenorhabditis elegans. In these models, both enzymes are required for H3 phosphorylation and chromosome segregation, although a causal link between the two processes has not been demonstrated. Deregulation of human aurora kinases has been implicated in oncogenesis as a consequence of chromosome missegregation. Our findings reveal an enzyme system that regulates chromosome dynamics and controls histone phosphorylation that is conserved among diverse eukaryotes.

Original languageEnglish (US)
Pages (from-to)279-291
Number of pages13
JournalCell
Volume102
Issue number3
StatePublished - Aug 4 2000

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Aurora Kinases
Phosphorylation
Saccharomycetales
Chromosomes
Phosphoric Monoester Hydrolases
Histones
Yeast
Eukaryota
Mitosis
Tetrahymena
Chromosome Segregation
Deregulation
Caenorhabditis elegans
Meiosis
Enzymes
Serine
Saccharomyces cerevisiae
Carcinogenesis

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Hsu, J. Y., Sun, Z. W., Li, X., Reuben, M., Tatchell, K., Bishop, D. K., ... Allis, C. D. (2000). Mitotic phosphorylation of histone H3 is governed by IpI1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. Cell, 102(3), 279-291.

Mitotic phosphorylation of histone H3 is governed by IpI1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. / Hsu, Jer Yuan; Sun, Zu Wen; Li, Xiumin; Reuben, Melanie; Tatchell, Kelly; Bishop, Douglas K.; Grushcow, Jeremy M.; Brame, Cynthia J.; Caldwell, Jennifer A.; Hunt, Donald F.; Lin, Rueyling; Smith, M. Mitchell; Allis, C. David.

In: Cell, Vol. 102, No. 3, 04.08.2000, p. 279-291.

Research output: Contribution to journalArticle

Hsu, JY, Sun, ZW, Li, X, Reuben, M, Tatchell, K, Bishop, DK, Grushcow, JM, Brame, CJ, Caldwell, JA, Hunt, DF, Lin, R, Smith, MM & Allis, CD 2000, 'Mitotic phosphorylation of histone H3 is governed by IpI1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes', Cell, vol. 102, no. 3, pp. 279-291.
Hsu JY, Sun ZW, Li X, Reuben M, Tatchell K, Bishop DK et al. Mitotic phosphorylation of histone H3 is governed by IpI1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. Cell. 2000 Aug 4;102(3):279-291.
Hsu, Jer Yuan ; Sun, Zu Wen ; Li, Xiumin ; Reuben, Melanie ; Tatchell, Kelly ; Bishop, Douglas K. ; Grushcow, Jeremy M. ; Brame, Cynthia J. ; Caldwell, Jennifer A. ; Hunt, Donald F. ; Lin, Rueyling ; Smith, M. Mitchell ; Allis, C. David. / Mitotic phosphorylation of histone H3 is governed by IpI1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. In: Cell. 2000 ; Vol. 102, No. 3. pp. 279-291.
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