@article{9c5c1a700b3141c38d7480cc6a35ece5,
title = "Mitotic phosphorylation of histone H3 is governed by IpI1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes",
abstract = "Phosphorylation of histone H3 at serine 10 occurs during mitosis and meiosis in a wide range of eukaryotes and has been shown to be required for proper chromosome transmission in Tetrahymena. Here we report that IpI1/aurora kinase and its genetically interacting phosphatase, GIc7/PP1, are responsible for the balance of H3 phosphorylation during mitosis in Saccharomyces cerevisiae and Caenorhabditis elegans. In these models, both enzymes are required for H3 phosphorylation and chromosome segregation, although a causal link between the two processes has not been demonstrated. Deregulation of human aurora kinases has been implicated in oncogenesis as a consequence of chromosome missegregation. Our findings reveal an enzyme system that regulates chromosome dynamics and controls histone phosphorylation that is conserved among diverse eukaryotes.",
author = "Hsu, {Jer Yuan} and Sun, {Zu Wen} and Xiumin Li and Melanie Reuben and Kelly Tatchell and Bishop, {Douglas K.} and Grushcow, {Jeremy M.} and Brame, {Cynthia J.} and Caldwell, {Jennifer A.} and Hunt, {Donald F.} and Rueyling Lin and Smith, {M. Mitchell} and Allis, {C. David}",
note = "Funding Information: We thank David Brautigan for providing mammalian PP1 and I-2, Craig Mizzen and Brian Strahl for assistance in preparation of chicken nucleosomes, Sepideh Khorazanizad for advice on expression and purification of recombinant proteins, Clarence Chan for providing yeast strains, Yuji Kohara for cDNA clones of air-1, air-2, and Ceglc-7, Jim Waddle for help with imaging, and the Caenorhabditis Genetic Center for worm strains used in this study. We also thank J. Waddle, Tim Schedl, Scott Robertson, and members of the Allis laboratory for discussions and critical reading of the manuscript. Z.-W. S. is supported by a postdoctoral cancer training grant from the University of Virginia Cancer Center. This research is supported by grants from the NIH to C. D. A. (GM40922), R. L. (HD37933), M. M. S. (GM28920), K. T. (GM47789), D. K. B. (GM50936), and D. F. H. (GM37537). ",
year = "2000",
month = aug,
day = "4",
doi = "10.1016/S0092-8674(00)00034-9",
language = "English (US)",
volume = "102",
pages = "279--291",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",
}