Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP)

Jianyu Wang, Xing Liu, Zhen Dou, Liang Chen, Hao Jiang, Chuanhai Fu, Guosheng Fu, Dan Liu, Jiancun Zhang, Tongge Zhu, Jingwen Fang, Jianye Zang, Jinke Cheng, Maikun Teng, Xia Ding, Xuebiao Yao

Research output: Contribution to journalArticle

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Abstract

The centromere is essential for precise and equal segregation of the parental genome into two daughter cells during mitosis. CENP-A is a unique histone H3 variant conserved in eukaryotic centromeres. The assembly of CENP-A to the centromere is mediated by Holliday junction recognition protein (HJURP) in early G1 phase. However, it remains elusive how HJURP governs CENP-A incorporation into the centromere. Here we show that human HJURP directly binds to Mis18β, a component of the Mis18 complex conserved in the eukaryotic kingdom. A minimal region of HJURP for Mis18β binding was mapped to residues 437-460. Depletion of Mis18β by RNA interference dramatically impaired HJURP recruitment to the centromere, indicating the importance of Mis18β in HJURP loading. Interestingly, phosphorylation of HJURP by CDK1 weakens its interaction with Mis18β, consistent with the notion that assembly of CENP-A to the centromere is achieved after mitosis. Taken together, these data define a novel molecular mechanism underlying the temporal regulation of CENP-A incorporation into the centromere by accurate Mis18β-HJURP interaction.

Original languageEnglish (US)
Pages (from-to)8326-8336
Number of pages11
JournalJournal of Biological Chemistry
Volume289
Issue number12
DOIs
StatePublished - Mar 21 2014

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Cruciform DNA
Molecular Chaperones
Centromere
Proteins
Mitosis
Phosphorylation
G1 Phase
RNA Interference
Protein Binding
Histones
Genes
centromere protein A
Genome
RNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP). / Wang, Jianyu; Liu, Xing; Dou, Zhen; Chen, Liang; Jiang, Hao; Fu, Chuanhai; Fu, Guosheng; Liu, Dan; Zhang, Jiancun; Zhu, Tongge; Fang, Jingwen; Zang, Jianye; Cheng, Jinke; Teng, Maikun; Ding, Xia; Yao, Xuebiao.

In: Journal of Biological Chemistry, Vol. 289, No. 12, 21.03.2014, p. 8326-8336.

Research output: Contribution to journalArticle

Wang, J, Liu, X, Dou, Z, Chen, L, Jiang, H, Fu, C, Fu, G, Liu, D, Zhang, J, Zhu, T, Fang, J, Zang, J, Cheng, J, Teng, M, Ding, X & Yao, X 2014, 'Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP)', Journal of Biological Chemistry, vol. 289, no. 12, pp. 8326-8336. https://doi.org/10.1074/jbc.M113.529958
Wang, Jianyu ; Liu, Xing ; Dou, Zhen ; Chen, Liang ; Jiang, Hao ; Fu, Chuanhai ; Fu, Guosheng ; Liu, Dan ; Zhang, Jiancun ; Zhu, Tongge ; Fang, Jingwen ; Zang, Jianye ; Cheng, Jinke ; Teng, Maikun ; Ding, Xia ; Yao, Xuebiao. / Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone holliday junction recognition protein (HJURP). In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 12. pp. 8326-8336.
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