Mitotic transcription and waves of gene reactivation during mitotic exit

Katherine C. Palozola, Greg Donahue, Hong Liu, Gregory R. Grant, Justin S. Becker, Allison Cote, Hongtao Yu, Arjun Raj, Kenneth S. Zaret

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Although the genome is generally thought to be transcriptionally silent during mitosis, technical limitations have prevented sensitive mapping of transcription during mitosis and mitotic exit. Thus, the means by which the interphase expression pattern is transduced to daughter cells have been unclear. We used 5-ethynyluridine to pulse-label transcripts during mitosis and mitotic exit and found that many genes exhibit transcription during mitosis, as confirmed with fluorescein isothiocyanate–uridine 5′-triphosphate labeling, RNA fluorescence in situ hybridization, and quantitative reverse transcription polymerase chain reaction. The first round of transcription immediately after mitosis primarily activates genes involved in the growth and rebuilding of daughter cells, rather than cell type–specific functions. We propose that the cell’s transcription pattern is largely retained at a low level through mitosis, whereas the amplitude of transcription observed in interphase is reestablished during mitotic exit.

Original languageEnglish (US)
Pages (from-to)119-122
Number of pages4
JournalScience
Volume358
Issue number6359
DOIs
StatePublished - Oct 6 2017

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Mitosis
Genes
Interphase
Fluorescein
Fluorescence In Situ Hybridization
Reverse Transcription
Genome
RNA
Polymerase Chain Reaction
Growth

ASJC Scopus subject areas

  • General

Cite this

Palozola, K. C., Donahue, G., Liu, H., Grant, G. R., Becker, J. S., Cote, A., ... Zaret, K. S. (2017). Mitotic transcription and waves of gene reactivation during mitotic exit. Science, 358(6359), 119-122. https://doi.org/10.1126/science.aal4671

Mitotic transcription and waves of gene reactivation during mitotic exit. / Palozola, Katherine C.; Donahue, Greg; Liu, Hong; Grant, Gregory R.; Becker, Justin S.; Cote, Allison; Yu, Hongtao; Raj, Arjun; Zaret, Kenneth S.

In: Science, Vol. 358, No. 6359, 06.10.2017, p. 119-122.

Research output: Contribution to journalArticle

Palozola, KC, Donahue, G, Liu, H, Grant, GR, Becker, JS, Cote, A, Yu, H, Raj, A & Zaret, KS 2017, 'Mitotic transcription and waves of gene reactivation during mitotic exit', Science, vol. 358, no. 6359, pp. 119-122. https://doi.org/10.1126/science.aal4671
Palozola KC, Donahue G, Liu H, Grant GR, Becker JS, Cote A et al. Mitotic transcription and waves of gene reactivation during mitotic exit. Science. 2017 Oct 6;358(6359):119-122. https://doi.org/10.1126/science.aal4671
Palozola, Katherine C. ; Donahue, Greg ; Liu, Hong ; Grant, Gregory R. ; Becker, Justin S. ; Cote, Allison ; Yu, Hongtao ; Raj, Arjun ; Zaret, Kenneth S. / Mitotic transcription and waves of gene reactivation during mitotic exit. In: Science. 2017 ; Vol. 358, No. 6359. pp. 119-122.
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