Mixed-lineage kinase 3 phosphorylates prolyl-isomerase Pin1 to regulate its nuclear translocation and cellular function

Velusamy Rangasamy, Rajakishore Mishra, Gautam Sondarva, Subhasis Das, Tae Ho Lee, Joanna C. Bakowska, Guri Tzivion, James S. Malter, Basabi Rana, Kun Ping Lu, Anumantha Kanthasamy, Ajay Rana

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Nuclear protein peptidyl-prolyl isomerase Pin1-mediated prolyl isomerization is an essential and novel regulatory mechanism for protein phosphorylation. Therefore, tight regulation of Pin1 localization and catalytic activity is crucial for its normal nuclear functions. Pin1 is commonly dysregulated during oncogenesis and likely contributes to these pathologies; however, the mechanism( s) by which Pin1 catalytic activity and nuclear localization are increased is unknown. Here we demonstrate that mixed-lineage kinase 3 (MLK3), a MAP3K family member, phosphorylates Pin1 on a Ser138 site to increase its catalytic activity and nuclear translocation. This phosphorylation event drives the cell cycle and promotes cyclin D1 stability and centrosome amplification. Notably, Pin1 pSer138 is significantly up-regulated in breast tumors and is localized in the nucleus. These findings collectively suggest that the MLK3-Pin1 signaling cascade plays a critical role in regulating the cell cycle, centrosome numbers, and oncogenesis.

Original languageEnglish (US)
Pages (from-to)8149-8154
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number21
DOIs
StatePublished - May 22 2012

Keywords

  • Breast cancer
  • JNK

ASJC Scopus subject areas

  • General

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    Rangasamy, V., Mishra, R., Sondarva, G., Das, S., Lee, T. H., Bakowska, J. C., Tzivion, G., Malter, J. S., Rana, B., Lu, K. P., Kanthasamy, A., & Rana, A. (2012). Mixed-lineage kinase 3 phosphorylates prolyl-isomerase Pin1 to regulate its nuclear translocation and cellular function. Proceedings of the National Academy of Sciences of the United States of America, 109(21), 8149-8154. https://doi.org/10.1073/pnas.1200804109