Modeling of diseases of retinal ischemia in vitro: Possible participation of autocrine vascular endothelial growth factor signaling

Ying Yan, Tao He, Yin Shen, Xiao Chen, Bo Diao, Zhi Li, Fang Zhou, Yi Qiao Xing

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: To establish and evaluate a novel in vitro model of retinal ischemia, and to determine whether an autocrine pathway of retinal microvascular endothelial cells (RMVECs) by vascular endothelial growth factor (VEGF) signaling plays a role based on this model. Methods: Primary RMVECs were isolated from the retinas of C57/BL6J rats and identified by an evaluation for FITC-marked CD31. The hypoxia models were established with the biobag at the time of 12, 24, 48 and 72 h, and evaluated with a blood-gas analyzer. The control groups were incubated under normoxic conditions for the same length of time. Cell proliferation was evaluated by the CCK-8 method. Apoptosis was assayed using a flow cytometry method. RNA and protein expressions of VEGF-A, VEGFR-2 and iNOs were analyzed by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Results: The results of blood-gas analysis showed that when the cultures were exposed to hypoxia for more than 2 h, the pO2 was below 4.5 kPa, pCO2 and pH shifted slightly. Real-time RT-PCR revealed that the expressions of VEGF-A, VEGFR-2 and iNOs mRNA in hypoxic groups increased in comparison to those in the normoxia groups (p < 0.01) and the expression of mRNA increased significantly in a time-dependent fashion in the hypoxic groups (p < 0.01), peaking at 48 h, and then decreasing. Western blot analysis revealed that the expression of relative proteins ranked in this order. CCK-8 analysis revealed that the proliferative capacity of RMVECs in the hypoxic groups was significantly higher than those in the normoxic groups at each time point (p < 0.05). At 48 h, the proliferative capacity was highest in the hypoxia groups (p < 0.05). Data acquisition from flow cytometry showed that cell survival rates in the hypoxic groups were higher than those in the normoxic groups and apoptosis rates dropped accordingly. The survival rate was highest at 48 h. Conclusion: These findings suggested that a novel in vitro model of retinal ischemia using the biobag had a good authenticity. According to the well-established in vitro hypoxia model by the biobag, RMVECs include the requisite elements for an autocrine pathway that may serve to amplify the angiogenic effects of VEGF.

Original languageEnglish (US)
Pages (from-to)90-99
Number of pages10
JournalOphthalmic Research
Volume49
Issue number2
DOIs
StatePublished - Jan 2013

Fingerprint

Retinal Diseases
Vascular Endothelial Growth Factor A
Ischemia
Endothelial Cells
Sincalide
Vascular Endothelial Growth Factor Receptor-2
Reverse Transcription
Flow Cytometry
Western Blotting
Apoptosis
Polymerase Chain Reaction
Messenger RNA
Blood Gas Analysis
Fluorescein-5-isothiocyanate
Retina
In Vitro Techniques
Cell Survival
Proteins
Gases
Cell Proliferation

Keywords

  • Autocrine
  • Hypoxia
  • Retinal microvascular endothelial cells

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Modeling of diseases of retinal ischemia in vitro : Possible participation of autocrine vascular endothelial growth factor signaling. / Yan, Ying; He, Tao; Shen, Yin; Chen, Xiao; Diao, Bo; Li, Zhi; Zhou, Fang; Xing, Yi Qiao.

In: Ophthalmic Research, Vol. 49, No. 2, 01.2013, p. 90-99.

Research output: Contribution to journalArticle

Yan, Ying ; He, Tao ; Shen, Yin ; Chen, Xiao ; Diao, Bo ; Li, Zhi ; Zhou, Fang ; Xing, Yi Qiao. / Modeling of diseases of retinal ischemia in vitro : Possible participation of autocrine vascular endothelial growth factor signaling. In: Ophthalmic Research. 2013 ; Vol. 49, No. 2. pp. 90-99.
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AU - Li, Zhi

AU - Zhou, Fang

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N2 - Purpose: To establish and evaluate a novel in vitro model of retinal ischemia, and to determine whether an autocrine pathway of retinal microvascular endothelial cells (RMVECs) by vascular endothelial growth factor (VEGF) signaling plays a role based on this model. Methods: Primary RMVECs were isolated from the retinas of C57/BL6J rats and identified by an evaluation for FITC-marked CD31. The hypoxia models were established with the biobag at the time of 12, 24, 48 and 72 h, and evaluated with a blood-gas analyzer. The control groups were incubated under normoxic conditions for the same length of time. Cell proliferation was evaluated by the CCK-8 method. Apoptosis was assayed using a flow cytometry method. RNA and protein expressions of VEGF-A, VEGFR-2 and iNOs were analyzed by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Results: The results of blood-gas analysis showed that when the cultures were exposed to hypoxia for more than 2 h, the pO2 was below 4.5 kPa, pCO2 and pH shifted slightly. Real-time RT-PCR revealed that the expressions of VEGF-A, VEGFR-2 and iNOs mRNA in hypoxic groups increased in comparison to those in the normoxia groups (p < 0.01) and the expression of mRNA increased significantly in a time-dependent fashion in the hypoxic groups (p < 0.01), peaking at 48 h, and then decreasing. Western blot analysis revealed that the expression of relative proteins ranked in this order. CCK-8 analysis revealed that the proliferative capacity of RMVECs in the hypoxic groups was significantly higher than those in the normoxic groups at each time point (p < 0.05). At 48 h, the proliferative capacity was highest in the hypoxia groups (p < 0.05). Data acquisition from flow cytometry showed that cell survival rates in the hypoxic groups were higher than those in the normoxic groups and apoptosis rates dropped accordingly. The survival rate was highest at 48 h. Conclusion: These findings suggested that a novel in vitro model of retinal ischemia using the biobag had a good authenticity. According to the well-established in vitro hypoxia model by the biobag, RMVECs include the requisite elements for an autocrine pathway that may serve to amplify the angiogenic effects of VEGF.

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