Modification of acute experimental autoimmune encephalomyelitis in the Lewis rat by oral administration of type 1 interferons

S. A. Brod, M. Scott, D. K. Burns, J. T. Phillips

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Abstract

The effect of orally administered type 1 interferons on the severity of acute experimental autoimmune encephalomyelitis (EAE), a T cell-mediated autoimmune disease, was examined by inoculation of Lewis rats with guinea pig myelin basic protein (GPMBP) and complete Freund's adjuvant. Rats were fed either rat species-specific or human recombinant type 1 interferon (IFN) or mock IFN daily for 7 days preceding immunization and for 21 days thereafter. There was a significant decrease in the clinical score and inflammatory foci in animals fed 5000 units IFN compared with mock-treated animals. There was a significant decrease in clinical score and number of inflammatory foci in spinal cord in animals fed orally 5000 units human recombinant IFN-α PO compared with SC 5000 units recombinant human IFN-α. Oral administration of type I interferon, as opposed to subcutaneous administration, inhibited the secretion of IFN-γ from ConA-activated draining popliteal lymph node cells compared with mock-fed animals. These experiments demonstrate that acute EAE is more effectively inhibited by equivalent amounts of orally in contrast to parenterally administered IFN-α. These results suggest that type 1 IFNs are active by the oral route and have significant clinical and histologic effects in acute autoimmune disease.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalJournal of Interferon and Cytokine Research
Volume15
Issue number2
StatePublished - 1995

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Interferon Type I
Autoimmune Experimental Encephalomyelitis
Interferons
Oral Administration
Autoimmune Diseases
Myelin Basic Protein
Freund's Adjuvant
Acute Disease
Immunization
Spinal Cord
Guinea Pigs
Lymph Nodes
T-Lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Virology

Cite this

Modification of acute experimental autoimmune encephalomyelitis in the Lewis rat by oral administration of type 1 interferons. / Brod, S. A.; Scott, M.; Burns, D. K.; Phillips, J. T.

In: Journal of Interferon and Cytokine Research, Vol. 15, No. 2, 1995, p. 115-122.

Research output: Contribution to journalArticle

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