Modification of subtelomeric DNA

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

There is a discrepancy in telomere length as measured by signal intensity of telomere restriction fragments on gels and fluorescence in situ hybridization analysis. This difference has been ascribed to the X-region, a segment of subtelomeric DNA that is resistant to being cut by restriction enzymes. To explore the nature of this region, we analyzed the digestibility of an artificial seeded telomere in HeLa cells as well as the Xp/Yp autosomal telomere in human BJ fibroblasts. We found that there is a substantial fraction of subtelomeric DNA containing restriction sites that is not digested with enzymes such as EcoRI, NIaIII, and SphI. Comparison of methylation-sensitive and -resistant enzymes excluded the possibility of the X-region being maintained by DNA methylation. We show that the X-region represents a variable domain whose size changes with telomere length, and neither non-TTAGGG sequences nor cytidine methylation can adequately explain the size of the X-region.

Original languageEnglish (US)
Pages (from-to)4571-4580
Number of pages10
JournalMolecular and Cellular Biology
Volume24
Issue number10
DOIs
StatePublished - May 2004

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Telomere
DNA
Methylation
Enzymes
Cytidine
DNA Methylation
Fluorescence In Situ Hybridization
HeLa Cells
Fibroblasts
Gels

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Modification of subtelomeric DNA. / Steinert, Susanne; Shay, Jerry W.; Wright, Woodring E.

In: Molecular and Cellular Biology, Vol. 24, No. 10, 05.2004, p. 4571-4580.

Research output: Contribution to journalArticle

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