Modular arrangement of allelic variants explains the divergence in moraxella catarrhalis uspa protein function

Michael J. Brooks, Jennifer L. Sedillo, Nikki Wagner, Cassie A. Laurence, Wei Wang, Ahmed S. Attia, Eric J. Hansen, Scott D. Gray-Owen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Ubiquitous surface protein A molecules (UspAs) of Moraxella catarrhalis are large, nonfimbrial, autotransporter proteins that can be visualized as a "fuzzy" layer on the bacterial surface by transmission electron microscopy. Previous studies attributed a wide array of functions and binding activities to the closely related UspA1, UspA2, and/or UspA2H protein, yet the molecular and phylogenetic relationships among these activities remain largely unexplored. To address this issue, we determined the nucleotide sequence of the uspA1 genes from a variety of independent M. catarrhalis isolates and compared the deduced amino acid sequences to those of the previously characterized UspA1, UspA2, and UspA2H proteins. Rather than being conserved proteins, we observed a striking divergence of individual UspA1, UspA2, and UspA2H proteins resulting from the modular assortment of unrelated "cassettes" of peptide sequence. The exchange of certain variant cassettes correlates with strain-specific differences in UspA protein function and confers differing phenotypes upon these mucosal surface pathogens.

Original languageEnglish (US)
Pages (from-to)5330-5340
Number of pages11
JournalInfection and Immunity
Volume76
Issue number11
DOIs
StatePublished - Nov 2008

Fingerprint

Moraxella (Branhamella) catarrhalis
Proteins
Staphylococcal Protein A
Transmission Electron Microscopy
Moraxella catarrhalis UspA protein
Amino Acid Sequence
Membrane Proteins
Phenotype
Peptides
Genes
Type V Secretion Systems

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Brooks, M. J., Sedillo, J. L., Wagner, N., Laurence, C. A., Wang, W., Attia, A. S., ... Gray-Owen, S. D. (2008). Modular arrangement of allelic variants explains the divergence in moraxella catarrhalis uspa protein function. Infection and Immunity, 76(11), 5330-5340. https://doi.org/10.1128/IAI.00573-08

Modular arrangement of allelic variants explains the divergence in moraxella catarrhalis uspa protein function. / Brooks, Michael J.; Sedillo, Jennifer L.; Wagner, Nikki; Laurence, Cassie A.; Wang, Wei; Attia, Ahmed S.; Hansen, Eric J.; Gray-Owen, Scott D.

In: Infection and Immunity, Vol. 76, No. 11, 11.2008, p. 5330-5340.

Research output: Contribution to journalArticle

Brooks, MJ, Sedillo, JL, Wagner, N, Laurence, CA, Wang, W, Attia, AS, Hansen, EJ & Gray-Owen, SD 2008, 'Modular arrangement of allelic variants explains the divergence in moraxella catarrhalis uspa protein function', Infection and Immunity, vol. 76, no. 11, pp. 5330-5340. https://doi.org/10.1128/IAI.00573-08
Brooks, Michael J. ; Sedillo, Jennifer L. ; Wagner, Nikki ; Laurence, Cassie A. ; Wang, Wei ; Attia, Ahmed S. ; Hansen, Eric J. ; Gray-Owen, Scott D. / Modular arrangement of allelic variants explains the divergence in moraxella catarrhalis uspa protein function. In: Infection and Immunity. 2008 ; Vol. 76, No. 11. pp. 5330-5340.
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