Modular assembly of chimeric phi29 packaging RNAs that support DNA packaging

Yun Fang, Dan Shu, Feng Xiao, Peixuan Guo, Peter Z. Qin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The bacteriophage phi29 DNA packaging motor is a protein/RNA complex that can produce strong force to condense the linear-double-stranded DNA genome into a pre-formed protein capsid. The RNA component, called the packaging RNA (pRNA), utilizes magnesium-dependent inter-molecular base-pairing interactions to form ring-shaped complexes. The pRNA is a class of non-coding RNA, interacting with phi29 motor proteins to enable DNA packaging. Here, we report a two-piece chimeric pRNA construct that is fully competent in interacting with partner pRNA to form ring-shaped complexes, in packaging DNA via the motor, and in assembling infectious phi29 virions in vitro. This is the first example of a fully functional pRNA assembled using two non-covalently interacting fragments. The results support the notion of modular pRNA architecture in the phi29 packaging motor.

Original languageEnglish (US)
Pages (from-to)589-594
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume372
Issue number4
DOIs
StatePublished - Aug 8 2008
Externally publishedYes

Fingerprint

DNA Packaging
Product Packaging
Packaging
RNA
DNA
Untranslated RNA
Capsid Proteins
Bacteriophages
Base Pairing
Virion
Magnesium
Proteins
Genome
Genes

Keywords

  • Bacteriophage
  • DNA packaging
  • Non-coding RNA
  • Packaging motor
  • Packaging RNA
  • phi29
  • RNA assembly

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Modular assembly of chimeric phi29 packaging RNAs that support DNA packaging. / Fang, Yun; Shu, Dan; Xiao, Feng; Guo, Peixuan; Qin, Peter Z.

In: Biochemical and Biophysical Research Communications, Vol. 372, No. 4, 08.08.2008, p. 589-594.

Research output: Contribution to journalArticle

Fang, Yun ; Shu, Dan ; Xiao, Feng ; Guo, Peixuan ; Qin, Peter Z. / Modular assembly of chimeric phi29 packaging RNAs that support DNA packaging. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 372, No. 4. pp. 589-594.
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