Modular 31 P wideband inversion transfer for integrative analysis of adenosine triphosphate metabolism, T 1 relaxation and molecular dynamics in skeletal muscle at 7T

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Abstract

Purpose: For efficient and integrative analysis of de novo adenosine triphosphate (ATP) synthesis, creatine-kinase–mediated ATP synthesis, T 1 relaxation time, and ATP molecular motion dynamics in human skeletal muscle at rest. Methods: Four inversion-transfer modules differing in center inversion frequency were combined to generate amplified magnetization transfer (MT) effects in targeted MT pathways, including Pi ↔ γ-ATP, PCr ↔ γ-ATP, and 31 P γ(α)ATP31 P βATP . MT effects from both forward and reverse exchange kinetic pathways were acquired to reduce potential bias and confounding factors in integrated data analysis. Results: Kinetic data collected using 4 wideband inversion modules (8 minutes each) yielded the forward exchange rate constants, k PCr →γ ATP = 0.31 ± 0.05 s –1 and k Pi →γ ATP = 0.064 ± 0.012 s –1 , and the reverse exchange rate constants, k γATP→Pi = 0.034 ± 0.006 s –1 and k γATP→PCr = 1.37 ± 0.22 s –1 , respectively. The cross-relaxation rate constant, σ γ(α) ↔ βATP was –0.20 ± 0.03 s –1 , corresponding to ATP rotational correlation time τ c of 0.8 ± 0.1 × 10 –7 seconds. The intrinsic T 1 relaxation times were Pi (9.2 ± 1.4 seconds), PCr (6.2 ± 0.4 seconds), γ-ATP (1.8 ± 0.1 seconds), α-ATP (1.4 ± 0.1 seconds), and β-ATP (1.1 ± 0.1 seconds). Muscle ATP T 1 values were found to be significantly longer than those previously measured in the brain using a similar method. Conclusion: A combination of multiple inversion transfer modules provides a comprehensive and integrated analysis of ATP metabolism and molecular motion dynamics. This relatively fast technique could be potentially useful for studying metabolic disorders in skeletal muscle.

Original languageEnglish (US)
JournalMagnetic resonance in medicine
DOIs
StatePublished - Jan 1 2019

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Molecular Dynamics Simulation
Skeletal Muscle
Adenosine Triphosphate
Creatine

Keywords

  • 31P MRS
  • ATP
  • brain
  • magnetization transfer
  • relaxation time
  • skeletal muscle

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

@article{5756d1522b3d4ce3af00b06ad55659a5,
title = "Modular 31 P wideband inversion transfer for integrative analysis of adenosine triphosphate metabolism, T 1 relaxation and molecular dynamics in skeletal muscle at 7T",
abstract = "Purpose: For efficient and integrative analysis of de novo adenosine triphosphate (ATP) synthesis, creatine-kinase–mediated ATP synthesis, T 1 relaxation time, and ATP molecular motion dynamics in human skeletal muscle at rest. Methods: Four inversion-transfer modules differing in center inversion frequency were combined to generate amplified magnetization transfer (MT) effects in targeted MT pathways, including Pi ↔ γ-ATP, PCr ↔ γ-ATP, and 31 P γ(α)ATP ↔ 31 P βATP . MT effects from both forward and reverse exchange kinetic pathways were acquired to reduce potential bias and confounding factors in integrated data analysis. Results: Kinetic data collected using 4 wideband inversion modules (8 minutes each) yielded the forward exchange rate constants, k PCr →γ ATP = 0.31 ± 0.05 s –1 and k Pi →γ ATP = 0.064 ± 0.012 s –1 , and the reverse exchange rate constants, k γATP→Pi = 0.034 ± 0.006 s –1 and k γATP→PCr = 1.37 ± 0.22 s –1 , respectively. The cross-relaxation rate constant, σ γ(α) ↔ βATP was –0.20 ± 0.03 s –1 , corresponding to ATP rotational correlation time τ c of 0.8 ± 0.1 × 10 –7 seconds. The intrinsic T 1 relaxation times were Pi (9.2 ± 1.4 seconds), PCr (6.2 ± 0.4 seconds), γ-ATP (1.8 ± 0.1 seconds), α-ATP (1.4 ± 0.1 seconds), and β-ATP (1.1 ± 0.1 seconds). Muscle ATP T 1 values were found to be significantly longer than those previously measured in the brain using a similar method. Conclusion: A combination of multiple inversion transfer modules provides a comprehensive and integrated analysis of ATP metabolism and molecular motion dynamics. This relatively fast technique could be potentially useful for studying metabolic disorders in skeletal muscle.",
keywords = "31P MRS, ATP, brain, magnetization transfer, relaxation time, skeletal muscle",
author = "Jimin Ren and Dean Sherry and Malloy, {Craig R}",
year = "2019",
month = "1",
day = "1",
doi = "10.1002/mrm.27686",
language = "English (US)",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Modular 31 P wideband inversion transfer for integrative analysis of adenosine triphosphate metabolism, T 1 relaxation and molecular dynamics in skeletal muscle at 7T

AU - Ren, Jimin

AU - Sherry, Dean

AU - Malloy, Craig R

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Purpose: For efficient and integrative analysis of de novo adenosine triphosphate (ATP) synthesis, creatine-kinase–mediated ATP synthesis, T 1 relaxation time, and ATP molecular motion dynamics in human skeletal muscle at rest. Methods: Four inversion-transfer modules differing in center inversion frequency were combined to generate amplified magnetization transfer (MT) effects in targeted MT pathways, including Pi ↔ γ-ATP, PCr ↔ γ-ATP, and 31 P γ(α)ATP ↔ 31 P βATP . MT effects from both forward and reverse exchange kinetic pathways were acquired to reduce potential bias and confounding factors in integrated data analysis. Results: Kinetic data collected using 4 wideband inversion modules (8 minutes each) yielded the forward exchange rate constants, k PCr →γ ATP = 0.31 ± 0.05 s –1 and k Pi →γ ATP = 0.064 ± 0.012 s –1 , and the reverse exchange rate constants, k γATP→Pi = 0.034 ± 0.006 s –1 and k γATP→PCr = 1.37 ± 0.22 s –1 , respectively. The cross-relaxation rate constant, σ γ(α) ↔ βATP was –0.20 ± 0.03 s –1 , corresponding to ATP rotational correlation time τ c of 0.8 ± 0.1 × 10 –7 seconds. The intrinsic T 1 relaxation times were Pi (9.2 ± 1.4 seconds), PCr (6.2 ± 0.4 seconds), γ-ATP (1.8 ± 0.1 seconds), α-ATP (1.4 ± 0.1 seconds), and β-ATP (1.1 ± 0.1 seconds). Muscle ATP T 1 values were found to be significantly longer than those previously measured in the brain using a similar method. Conclusion: A combination of multiple inversion transfer modules provides a comprehensive and integrated analysis of ATP metabolism and molecular motion dynamics. This relatively fast technique could be potentially useful for studying metabolic disorders in skeletal muscle.

AB - Purpose: For efficient and integrative analysis of de novo adenosine triphosphate (ATP) synthesis, creatine-kinase–mediated ATP synthesis, T 1 relaxation time, and ATP molecular motion dynamics in human skeletal muscle at rest. Methods: Four inversion-transfer modules differing in center inversion frequency were combined to generate amplified magnetization transfer (MT) effects in targeted MT pathways, including Pi ↔ γ-ATP, PCr ↔ γ-ATP, and 31 P γ(α)ATP ↔ 31 P βATP . MT effects from both forward and reverse exchange kinetic pathways were acquired to reduce potential bias and confounding factors in integrated data analysis. Results: Kinetic data collected using 4 wideband inversion modules (8 minutes each) yielded the forward exchange rate constants, k PCr →γ ATP = 0.31 ± 0.05 s –1 and k Pi →γ ATP = 0.064 ± 0.012 s –1 , and the reverse exchange rate constants, k γATP→Pi = 0.034 ± 0.006 s –1 and k γATP→PCr = 1.37 ± 0.22 s –1 , respectively. The cross-relaxation rate constant, σ γ(α) ↔ βATP was –0.20 ± 0.03 s –1 , corresponding to ATP rotational correlation time τ c of 0.8 ± 0.1 × 10 –7 seconds. The intrinsic T 1 relaxation times were Pi (9.2 ± 1.4 seconds), PCr (6.2 ± 0.4 seconds), γ-ATP (1.8 ± 0.1 seconds), α-ATP (1.4 ± 0.1 seconds), and β-ATP (1.1 ± 0.1 seconds). Muscle ATP T 1 values were found to be significantly longer than those previously measured in the brain using a similar method. Conclusion: A combination of multiple inversion transfer modules provides a comprehensive and integrated analysis of ATP metabolism and molecular motion dynamics. This relatively fast technique could be potentially useful for studying metabolic disorders in skeletal muscle.

KW - 31P MRS

KW - ATP

KW - brain

KW - magnetization transfer

KW - relaxation time

KW - skeletal muscle

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