Modulating Ca²⁺ in radiation-induced apoptosis suppresses DNA fragmentation but does not enhance clonogenic survival

The role of intracellular Ca²⁺ in radiation-induced apoptosis was studied:in a cell line derived from a mouse B-cell lymphoma (LY-TH). These cells had previously been shown to be sensitive to radiation and to die by apoptosis. The cell permeant Ca²⁺ chelator (acetyoxymethyl-)-1,2-bis(o-aminophenoxy)ethane-N,N,N:N'-tetraacetic acid (BAPTA/AM) reduced the DNA fragmentation characteristic of apoptosis but had no effect on clonogenic survival, Intracellular Ca²⁺ concentrations measured using the fluorescent indicator fura-2 only slowly increased over control values after cells were irradiated unlike the rapid increase observed in other systems. Our results indicate that modulating the endpoint of DNA fragmentation using some agents may not necessarily alter the cells' commitment to death as determined by clonogenic survival assays. This suggests that such agents play a role downstream of early initiation steps in apoptosis and modulate only particular features of apoptosis after the cell is committed to die.