Modulating Ca2+ in radiation-induced apoptosis suppresses DNA fragmentation but does not enhance clonogenic survival

D. W. Voehringer, M. D. Story, R. G. O'Neil, R. E. Meyn

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The role of intracellular Ca2+ in radiation-induced apoptosis was studied:in a cell line derived from a mouse B-cell lymphoma (LY-TH). These cells had previously been shown to be sensitive to radiation and to die by apoptosis. The cell permeant Ca2+ chelator (acetyoxymethyl-)-1,2-bis(o-aminophenoxy)ethane-N,N,N:N'-tetraacetic acid (BAPTA/AM) reduced the DNA fragmentation characteristic of apoptosis but had no effect on clonogenic survival, Intracellular Ca2+ concentrations measured using the fluorescent indicator fura-2 only slowly increased over control values after cells were irradiated unlike the rapid increase observed in other systems. Our results indicate that modulating the endpoint of DNA fragmentation using some agents may not necessarily alter the cells' commitment to death as determined by clonogenic survival assays. This suggests that such agents play a role downstream of early initiation steps in apoptosis and modulate only particular features of apoptosis after the cell is committed to die.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalInternational Journal of Radiation Biology
Volume71
Issue number3
DOIs
StatePublished - 1997

Fingerprint

DNA fragmentation
apoptosis
DNA Fragmentation
Cell death
fragmentation
DNA
deoxyribonucleic acid
Radiation
Apoptosis
calcium
radiation
cells
Cells
ethane
Ethane
Fura-2
B-Cell Lymphoma
Chelating Agents
chelating agents
lymphoma

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Modulating Ca2+ in radiation-induced apoptosis suppresses DNA fragmentation but does not enhance clonogenic survival. / Voehringer, D. W.; Story, M. D.; O'Neil, R. G.; Meyn, R. E.

In: International Journal of Radiation Biology, Vol. 71, No. 3, 1997, p. 237-243.

Research output: Contribution to journalArticle

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