Abstract
In bacterial sepsis and meningitis, large concentrations of interleukin-1β (IL-1β and tumor necrosis factor-α (TNF-α correlate directly with morbidity and mortality. This laboratory has reported previously that elevated temperature in the physiologic range is associated with down regulation of IL-1β and TNFα expression in cultured astroglia after lipopolysaccharide (LPS) stimulation. To further investigate the role of elevated temperature in the CNS inflammatory response, the effects of LPS and elevated temperature on the expression of genes that participate in the inflammatory response were determined in cultured transformed human fetal astrocytes and in an astrocytoma cell line. The effect of physiologic temperature elevation on cytokine concentrations in cerebrospinal fluid (CSF) was also investigated in a rabbit meningitis model. The findings indicate that astrocytes express a wide variety of cytokines, growth factors, growth factor receptors, and other genes that could play important roles in CNS inflammation. Furthermore, temperature elevation in the febrile range can lead to alterations in the patterns of expression of many genes involved in the inflammatory response of these cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 775-785 |
| Number of pages | 11 |
| Journal | Immunological Investigations |
| Volume | 24 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1995 |
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ASJC Scopus subject areas
- Immunology
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Modulation of expression of genes involved in the inflammatory response by lipopolysaccharide and temperature in cultured human astroglial cells. / Kimberlin, David W.; Velasco, Susana; Paris, Maria M.; Hickey, Sheila M.; McCracken, George H.; Nisen, Perry D.
In: Immunological Investigations, Vol. 24, No. 5, 1995, p. 775-785.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulation of expression of genes involved in the inflammatory response by lipopolysaccharide and temperature in cultured human astroglial cells
AU - Kimberlin, David W.
AU - Velasco, Susana
AU - Paris, Maria M.
AU - Hickey, Sheila M.
AU - McCracken, George H.
AU - Nisen, Perry D.
PY - 1995
Y1 - 1995
N2 - In bacterial sepsis and meningitis, large concentrations of interleukin-1β (IL-1β and tumor necrosis factor-α (TNF-α correlate directly with morbidity and mortality. This laboratory has reported previously that elevated temperature in the physiologic range is associated with down regulation of IL-1β and TNFα expression in cultured astroglia after lipopolysaccharide (LPS) stimulation. To further investigate the role of elevated temperature in the CNS inflammatory response, the effects of LPS and elevated temperature on the expression of genes that participate in the inflammatory response were determined in cultured transformed human fetal astrocytes and in an astrocytoma cell line. The effect of physiologic temperature elevation on cytokine concentrations in cerebrospinal fluid (CSF) was also investigated in a rabbit meningitis model. The findings indicate that astrocytes express a wide variety of cytokines, growth factors, growth factor receptors, and other genes that could play important roles in CNS inflammation. Furthermore, temperature elevation in the febrile range can lead to alterations in the patterns of expression of many genes involved in the inflammatory response of these cells.
AB - In bacterial sepsis and meningitis, large concentrations of interleukin-1β (IL-1β and tumor necrosis factor-α (TNF-α correlate directly with morbidity and mortality. This laboratory has reported previously that elevated temperature in the physiologic range is associated with down regulation of IL-1β and TNFα expression in cultured astroglia after lipopolysaccharide (LPS) stimulation. To further investigate the role of elevated temperature in the CNS inflammatory response, the effects of LPS and elevated temperature on the expression of genes that participate in the inflammatory response were determined in cultured transformed human fetal astrocytes and in an astrocytoma cell line. The effect of physiologic temperature elevation on cytokine concentrations in cerebrospinal fluid (CSF) was also investigated in a rabbit meningitis model. The findings indicate that astrocytes express a wide variety of cytokines, growth factors, growth factor receptors, and other genes that could play important roles in CNS inflammation. Furthermore, temperature elevation in the febrile range can lead to alterations in the patterns of expression of many genes involved in the inflammatory response of these cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029146425&partnerID=8YFLogxK
U2 - 10.3109/08820139509060705
DO - 10.3109/08820139509060705
M3 - Article
C2 - 8543341
AN - SCOPUS:0029146425
VL - 24
SP - 775
EP - 785
JO - Immunological Investigations
JF - Immunological Investigations
SN - 0882-0139
IS - 5
ER -