Modulation of hepatic transcriptome in the poloxamer p-407 hyperlipidemia mouse model

Drago Kuzman, Klementina Fon Tacer, M. Cerne, Tadeja Reen, Jure Aimovi, Urka Cegovnik, Darko Kocjan, Uros Urleb, Damjana Rozman

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The poloxamer P-407 hyperlipidemia mouse is a genetically unaltered, nondiet-induced model of atherosclerosis which is useful in studying effects of lipid lowering drugs. While the model is relatively well described on the biochemical level, the knowledge regarding the global gene expression was lacking so far. As expected, the P-407 elevates plasma triglycerides and cholesterol. Using microarray analysis and RT-PCR we show for the first time that poloxamer triggers a concerted modulation of hepatic genes involved in multiple steps of atherogenesis, such as lipid metabolism, initiation of atherogenesis, lesion formation, and atheroma progression. 106 hepatic genes were upregulated and only 40 downregulated, suggesting that upregulation of atherogenic genes precedes the atheroma formation. Modulated expression of circadian genes has also been observed in this type of hyperlipidemia but further studies are needed to elucidate whether this effects the rhythm of animals.

Original languageEnglish (US)
Pages (from-to)262-269
Number of pages8
JournalActa Chimica Slovenica
Volume56
Issue number1
StatePublished - Apr 30 2009

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Keywords

  • Atherogenesis
  • Circadian
  • Hyperlipidemia
  • Poloxamer

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Kuzman, D., Tacer, K. F., Cerne, M., Reen, T., Aimovi, J., Cegovnik, U., Kocjan, D., Urleb, U., & Rozman, D. (2009). Modulation of hepatic transcriptome in the poloxamer p-407 hyperlipidemia mouse model. Acta Chimica Slovenica, 56(1), 262-269.