Modulation of Rac Localization and Function by Dynamin

Günther Schlunck, Hanna Damke, William B. Kiosses, Nicole Rusk, Marc H. Symons, Clare M. Waterman-Storer, Sandra L. Schmid, Martin Alexander Schwartz

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The GTPase dynamin controls a variety of endocytic pathways, participates in the formation of phagosomes, podosomal adhesions, and invadopodia, and in regulation of the cytoskeleton and apoptosis. Rac, a member of the Rho family of small GTPases, controls formation of lamellipodia and focal complexes, which are critical in cell migration and phagocytosis. We now show that disruption of dynamin-2 function alters Rac localization and inhibits cell spreading and lamellipodia formation even though Rac is activated. Dominant-negative K44A dynamin-2 inhibited cell spreading and lamellipodia formation on fibronectin without blocking cell adhesion; dynamin-2 depletion by specific small interfering RNA inhibited lamellipodia in a similar manner. Dyn2(K44A) induced Rac mislocalization away from cell edges, into abnormal dorsal ruffles, and led to increased total Rac activity. Fluorescence resonance energy transfer imaging of Rac activity confirmed its predominant localization to aberrant dorsal ruffles in the presence of dominant-negative dyn2(K44A). Dyn2(K44A) induced the accumulation of tubulated structures bearing membrane-bound Rac-GFP. Constitutively active but not wild-type GFP-Rac was found on macropinosomes and Rac-dependent, platelet-derived growth factor-induced macropinocytosis was abolished by Dyn2(K44A) expression. These data suggest an indispensable role of dynamin in Rac trafficking to allow for lamellipodia formation and cell spreading.

Original languageEnglish (US)
Pages (from-to)256-267
Number of pages12
JournalMolecular Biology of the Cell
Volume15
Issue number1
DOIs
StatePublished - Jan 2004

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Dynamins
Pseudopodia
Dynamin II
Cytophagocytosis
Phagosomes
Fluorescence Resonance Energy Transfer
Monomeric GTP-Binding Proteins
GTP Phosphohydrolases
Platelet-Derived Growth Factor
Cytoskeleton
Fibronectins
Cell Adhesion
Small Interfering RNA
Cell Movement
Apoptosis
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Schlunck, G., Damke, H., Kiosses, W. B., Rusk, N., Symons, M. H., Waterman-Storer, C. M., ... Schwartz, M. A. (2004). Modulation of Rac Localization and Function by Dynamin. Molecular Biology of the Cell, 15(1), 256-267. https://doi.org/10.1091/mbc.E03-01-0019

Modulation of Rac Localization and Function by Dynamin. / Schlunck, Günther; Damke, Hanna; Kiosses, William B.; Rusk, Nicole; Symons, Marc H.; Waterman-Storer, Clare M.; Schmid, Sandra L.; Schwartz, Martin Alexander.

In: Molecular Biology of the Cell, Vol. 15, No. 1, 01.2004, p. 256-267.

Research output: Contribution to journalArticle

Schlunck, G, Damke, H, Kiosses, WB, Rusk, N, Symons, MH, Waterman-Storer, CM, Schmid, SL & Schwartz, MA 2004, 'Modulation of Rac Localization and Function by Dynamin', Molecular Biology of the Cell, vol. 15, no. 1, pp. 256-267. https://doi.org/10.1091/mbc.E03-01-0019
Schlunck G, Damke H, Kiosses WB, Rusk N, Symons MH, Waterman-Storer CM et al. Modulation of Rac Localization and Function by Dynamin. Molecular Biology of the Cell. 2004 Jan;15(1):256-267. https://doi.org/10.1091/mbc.E03-01-0019
Schlunck, Günther ; Damke, Hanna ; Kiosses, William B. ; Rusk, Nicole ; Symons, Marc H. ; Waterman-Storer, Clare M. ; Schmid, Sandra L. ; Schwartz, Martin Alexander. / Modulation of Rac Localization and Function by Dynamin. In: Molecular Biology of the Cell. 2004 ; Vol. 15, No. 1. pp. 256-267.
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