Molecular and cellular heterogeneity of Wilms' tumor

S. Velasco, D. D'Amico, N. R. Schneider, C. Timmons, E. Chappell, D. Lee, P. D. Nisen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We developed a Wilms' tumor-cell culture system to investigate the molecular basis of nephrogenesis and oncogenesis. Several distinct fractions of cells were isolated and characterized from the same tumor specimen. The cells exhibited striking differences in morphology, immunocytochemical staining profiles and cytogenetics. One fraction contained cells with features of epithelium; other cell fractions resembled partially differentiated mesenchyme (blastema or stroma). While the Wilms' tumor-suppressor gene WT1 was not altered, loss of heterozygosity (LOH) and an insertion in intron 1 of the p53 tumor-suppressor gene occurred in the tumor and the cultured cell types. LOH for RB was detected only in the cultured cells. These findings are consistent with a model of tumor initiation in a pluripotent cell that is able to undergo subsequent differentiation along multiple different lines and which mimics normal nephrogenesis.

Original languageEnglish (US)
Pages (from-to)672-679
Number of pages8
JournalInternational Journal of Cancer
Volume53
Issue number4
StatePublished - 1993

Fingerprint

Wilms Tumor
Loss of Heterozygosity
Tumor Suppressor Genes
Wilms' Tumor Genes
Cultured Tumor Cells
Mesoderm
Cytogenetics
Introns
Cultured Cells
Neoplasms
Carcinogenesis
Epithelium
Cell Culture Techniques
Staining and Labeling

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Velasco, S., D'Amico, D., Schneider, N. R., Timmons, C., Chappell, E., Lee, D., & Nisen, P. D. (1993). Molecular and cellular heterogeneity of Wilms' tumor. International Journal of Cancer, 53(4), 672-679.

Molecular and cellular heterogeneity of Wilms' tumor. / Velasco, S.; D'Amico, D.; Schneider, N. R.; Timmons, C.; Chappell, E.; Lee, D.; Nisen, P. D.

In: International Journal of Cancer, Vol. 53, No. 4, 1993, p. 672-679.

Research output: Contribution to journalArticle

Velasco, S, D'Amico, D, Schneider, NR, Timmons, C, Chappell, E, Lee, D & Nisen, PD 1993, 'Molecular and cellular heterogeneity of Wilms' tumor', International Journal of Cancer, vol. 53, no. 4, pp. 672-679.
Velasco S, D'Amico D, Schneider NR, Timmons C, Chappell E, Lee D et al. Molecular and cellular heterogeneity of Wilms' tumor. International Journal of Cancer. 1993;53(4):672-679.
Velasco, S. ; D'Amico, D. ; Schneider, N. R. ; Timmons, C. ; Chappell, E. ; Lee, D. ; Nisen, P. D. / Molecular and cellular heterogeneity of Wilms' tumor. In: International Journal of Cancer. 1993 ; Vol. 53, No. 4. pp. 672-679.
@article{5580d97017a841f1a4c0952a0945600a,
title = "Molecular and cellular heterogeneity of Wilms' tumor",
abstract = "We developed a Wilms' tumor-cell culture system to investigate the molecular basis of nephrogenesis and oncogenesis. Several distinct fractions of cells were isolated and characterized from the same tumor specimen. The cells exhibited striking differences in morphology, immunocytochemical staining profiles and cytogenetics. One fraction contained cells with features of epithelium; other cell fractions resembled partially differentiated mesenchyme (blastema or stroma). While the Wilms' tumor-suppressor gene WT1 was not altered, loss of heterozygosity (LOH) and an insertion in intron 1 of the p53 tumor-suppressor gene occurred in the tumor and the cultured cell types. LOH for RB was detected only in the cultured cells. These findings are consistent with a model of tumor initiation in a pluripotent cell that is able to undergo subsequent differentiation along multiple different lines and which mimics normal nephrogenesis.",
author = "S. Velasco and D. D'Amico and Schneider, {N. R.} and C. Timmons and E. Chappell and D. Lee and Nisen, {P. D.}",
year = "1993",
language = "English (US)",
volume = "53",
pages = "672--679",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Molecular and cellular heterogeneity of Wilms' tumor

AU - Velasco, S.

AU - D'Amico, D.

AU - Schneider, N. R.

AU - Timmons, C.

AU - Chappell, E.

AU - Lee, D.

AU - Nisen, P. D.

PY - 1993

Y1 - 1993

N2 - We developed a Wilms' tumor-cell culture system to investigate the molecular basis of nephrogenesis and oncogenesis. Several distinct fractions of cells were isolated and characterized from the same tumor specimen. The cells exhibited striking differences in morphology, immunocytochemical staining profiles and cytogenetics. One fraction contained cells with features of epithelium; other cell fractions resembled partially differentiated mesenchyme (blastema or stroma). While the Wilms' tumor-suppressor gene WT1 was not altered, loss of heterozygosity (LOH) and an insertion in intron 1 of the p53 tumor-suppressor gene occurred in the tumor and the cultured cell types. LOH for RB was detected only in the cultured cells. These findings are consistent with a model of tumor initiation in a pluripotent cell that is able to undergo subsequent differentiation along multiple different lines and which mimics normal nephrogenesis.

AB - We developed a Wilms' tumor-cell culture system to investigate the molecular basis of nephrogenesis and oncogenesis. Several distinct fractions of cells were isolated and characterized from the same tumor specimen. The cells exhibited striking differences in morphology, immunocytochemical staining profiles and cytogenetics. One fraction contained cells with features of epithelium; other cell fractions resembled partially differentiated mesenchyme (blastema or stroma). While the Wilms' tumor-suppressor gene WT1 was not altered, loss of heterozygosity (LOH) and an insertion in intron 1 of the p53 tumor-suppressor gene occurred in the tumor and the cultured cell types. LOH for RB was detected only in the cultured cells. These findings are consistent with a model of tumor initiation in a pluripotent cell that is able to undergo subsequent differentiation along multiple different lines and which mimics normal nephrogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0027513433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027513433&partnerID=8YFLogxK

M3 - Article

VL - 53

SP - 672

EP - 679

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -