Molecular approaches to the study of nucleotide excision repair in eukaryotes.

E. C. Friedberg, D. P. Barbis, J. M. Chenevert, R. Fleer, D. Kalainov, L. Naumovski, C. M. Nicolet, G. W. Robinson, R. A. Schultz, W. A. Weiss

Research output: Contribution to journalArticle

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Abstract

Very little is known about the molecular mechanism of nucleotide excision repair in eukaryotes. Studies on human cells have been stimulated by the availability of excision repair-defective cell lines from patients suffering from the autosomal recessive disease xeroderma pigmentosum (XP). Such studies have contributed significantly to an understanding of the genetic complexity of excision repair in human cells. However, to date, no human excision repair genes or gene products known to complement the repair defect in XP cells have been isolated. The yeast Saccharomyces cerevisiae is an interesting model for exploring the molecular mechanism of nucleotide excision repair in eukaryotic cells. As is true in human cells, multiple yeast genes are involved and at least five genes are required for the specific incision of UV-irradiated DNA in vivo. These five genes have been isolated by molecular cloning and the nucleotide sequences of four of them have been determined. Each of these cloned genes is being used for overexpression of protein.

Original languageEnglish (US)
Pages (from-to)311-318
Number of pages8
JournalBasic Life Sciences
Volume38
StatePublished - 1986

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Eukaryota
DNA Repair
Genes
Xeroderma Pigmentosum
Yeasts
Molecular Models
Molecular Cloning
Eukaryotic Cells
Saccharomyces cerevisiae
Cell Line
DNA
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Friedberg, E. C., Barbis, D. P., Chenevert, J. M., Fleer, R., Kalainov, D., Naumovski, L., ... Weiss, W. A. (1986). Molecular approaches to the study of nucleotide excision repair in eukaryotes. Basic Life Sciences, 38, 311-318.

Molecular approaches to the study of nucleotide excision repair in eukaryotes. / Friedberg, E. C.; Barbis, D. P.; Chenevert, J. M.; Fleer, R.; Kalainov, D.; Naumovski, L.; Nicolet, C. M.; Robinson, G. W.; Schultz, R. A.; Weiss, W. A.

In: Basic Life Sciences, Vol. 38, 1986, p. 311-318.

Research output: Contribution to journalArticle

Friedberg, EC, Barbis, DP, Chenevert, JM, Fleer, R, Kalainov, D, Naumovski, L, Nicolet, CM, Robinson, GW, Schultz, RA & Weiss, WA 1986, 'Molecular approaches to the study of nucleotide excision repair in eukaryotes.', Basic Life Sciences, vol. 38, pp. 311-318.
Friedberg EC, Barbis DP, Chenevert JM, Fleer R, Kalainov D, Naumovski L et al. Molecular approaches to the study of nucleotide excision repair in eukaryotes. Basic Life Sciences. 1986;38:311-318.
Friedberg, E. C. ; Barbis, D. P. ; Chenevert, J. M. ; Fleer, R. ; Kalainov, D. ; Naumovski, L. ; Nicolet, C. M. ; Robinson, G. W. ; Schultz, R. A. ; Weiss, W. A. / Molecular approaches to the study of nucleotide excision repair in eukaryotes. In: Basic Life Sciences. 1986 ; Vol. 38. pp. 311-318.
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