Molecular basis for cohesin acetylation by establishment of sister chromatid cohesion N-acetyltransferase ESCO1

Yadilette Rivera-Colón, Andrew Maguire, Glen P. Liszczak, Adam S. Olia, Ronen Marmorstein

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Protein acetylation is a prevalent posttranslational modification that is regulated by diverse acetyltransferase enzymes. Although histone acetyltransferases (HATs) have been well characterized both structurally and mechanistically, far less is known about non-histone acetyltransferase enzymes. The human ESCO1 and ESCO2 paralogs acetylate the cohesin complex subunit SMC3 to regulate the separation of sister chromatids during mitosis and meiosis. Missense mutations within the acetyltransferase domain of these proteins correlate with diseases, including endometrial cancers and Roberts syndrome. Despite their biological importance, the mechanisms underlying acetylation by the ESCO proteins are not understood. Here, we report the X-ray crystal structure of the highly conserved zinc finger-acetyltransferase moiety of ESCO1with accompanying structure-based mutagenesis and biochemical characterization. We find that the ESCO1 acetyltransferase core is structurally homologous to the Gcn5 HAT, but contains unique additional features including a zinc finger and an ~40-residue loop region that appear to play roles in protein stability and SMC3 substrate binding. We identify key residues that play roles in substrate binding and catalysis, and rationalize the functional consequences of disease-associated mutations. Together, these studies reveal the molecular basis for SMC3 acetylation by ESCO1 and have broader implications for understanding the structure/function of non-histone acetyltransferases.

Original languageEnglish (US)
Pages (from-to)26468-26477
Number of pages10
JournalJournal of Biological Chemistry
Volume291
Issue number51
DOIs
StatePublished - Dec 16 2016
Externally publishedYes

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Acetylation
Acetyltransferases
Chromatids
Histone Acetyltransferases
Zinc Fingers
Zinc
Proteins
Mutagenesis
Protein Stability
Meiosis
Missense Mutation
Substrates
Enzymes
Post Translational Protein Processing
Endometrial Neoplasms
Catalysis
Mitosis
cohesins
Crystal structure
X-Rays

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular basis for cohesin acetylation by establishment of sister chromatid cohesion N-acetyltransferase ESCO1. / Rivera-Colón, Yadilette; Maguire, Andrew; Liszczak, Glen P.; Olia, Adam S.; Marmorstein, Ronen.

In: Journal of Biological Chemistry, Vol. 291, No. 51, 16.12.2016, p. 26468-26477.

Research output: Contribution to journalArticle

Rivera-Colón, Yadilette ; Maguire, Andrew ; Liszczak, Glen P. ; Olia, Adam S. ; Marmorstein, Ronen. / Molecular basis for cohesin acetylation by establishment of sister chromatid cohesion N-acetyltransferase ESCO1. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 51. pp. 26468-26477.
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