Molecular characterization of a membrane transporter for lactate, pyruvate, and other monocarboxylates: Implications for the Cori cycle

Christine Kim Garcia, Joseph L. Goldstein, Ravindra K. Pathak, Richard G W Anderson, Michael S. Brown

Research output: Contribution to journalArticlepeer-review

409 Scopus citations

Abstract

Lactate and pyruvate cross cell membranes via a monocarboxylate transporter (MCT) with well-defined properties but undefined molecular structure. We report the cloning of a cDNA encoding MCT1, a monocarboxylate transporter whose properties resemble those of the erythrocyte MCT, including proton symport, trans acceleration, and sensitivity to α-cyanocinammates. A Phe to Cys substitution in MCT1 converts it to Mev, a mevalonate transporter. MCT1 is abundant in erythrocytes, cardiac muscle, and basolateral intestinal epithelium. In skeletal muscle it is restricted to mitochondria-rich myocytes. As sperm traverse the epididymis, MCT1 switches from sperm to epithelial cells. MCT1 is present at low levels in liver, suggesting another MCT in this tissue. By exporting lactate from intestine and erythrocytes, MCT1 participates in the Cori cycle. It also participates in novel pathways of monocarboxylate metabolism in muscle and sperm.

Original languageEnglish (US)
Pages (from-to)865-873
Number of pages9
JournalCell
Volume76
Issue number5
DOIs
StatePublished - Mar 11 1994

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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