Molecular Characterization of the Cellular Receptor for Poliovirus

Günter Bernhardt, James A. Bibb, Jonathan Bradley, Eckard Wimmer

Research output: Contribution to journalArticle

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Abstract

The expression of the human poliovirus receptor (hPVR) in several cultured cell lines was studied with the use of different antibodies directed against hPVR proteins. Immunoprecipitations of metabolically labeled cell lysates revealed that membrane-bound glycoforms of hPVR proteins have a molecular weight of about 80 kDa. By applying inhibitors of the glycosylation pathway (deoxymannojirimycin and swainsonine) we were able to monitor the modification of the hPVR glycoproteins when passing through the processing pathway. We show that a 67-kDa hPVR protein identified earlier (using a vaccinia virus expression system) is an intermediate glycoform probably located in the endoplasmic reticulum or cis-Golgi. Further modification of this glycoform is blocked by vaccinia virus infection or by the inhibitor deoxymannojirimycin. We, therefore, conclude that the 80-kDa glycoforms identified here are the fully processed hPVR isoforms. Surface iodination confirms that only the 80-kDa glycoforms are expressed on the cell surface. Treatment with various deglycosylating enzymes (N-Glycanase, O-Glycanase, Endo-H, and neuraminidase) demonstrates that the hPVR proteins bear sialylated complex-type oligosaccharides. Deglycosylation of the hPVR proteins also reveals the presence of both hPVR membrane-bound forms in cultured cells. Their relative expression levels, with respect to each other, vary considerably. The distribution of these hPVR isoforms in tissues may help explain the natural function of the hPVR proteins.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalVirology
Volume199
Issue number1
DOIs
StatePublished - Feb 15 1994

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Proteins
Vaccinia virus
Cultured Cells
Protein Isoforms
poliovirus receptor
Swainsonine
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Membranes
Halogenation
Neuraminidase
Virus Diseases
Oligosaccharides
Glycosylation
Immunoprecipitation
Endoplasmic Reticulum
Glycoproteins
Molecular Weight
Cell Line
Antibodies
Enzymes

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Molecular Characterization of the Cellular Receptor for Poliovirus. / Bernhardt, Günter; Bibb, James A.; Bradley, Jonathan; Wimmer, Eckard.

In: Virology, Vol. 199, No. 1, 15.02.1994, p. 105-113.

Research output: Contribution to journalArticle

Bernhardt, Günter ; Bibb, James A. ; Bradley, Jonathan ; Wimmer, Eckard. / Molecular Characterization of the Cellular Receptor for Poliovirus. In: Virology. 1994 ; Vol. 199, No. 1. pp. 105-113.
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