Molecular cloning and characterization of an amphibian progesterone receptor from Rana dybowskii

Li Wang, Sabyasachi Sanyal, Dayoung Oh, Joon Young Kim, Jung Won Ju, Kwang Hoon Song, Jung Woo Kim, Hyuk Bang Kwon, Hueng Sik Choi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Progesterone plays a pivotal role in the regulation of reproduction in all vertebrates and binds to nuclear hormone receptor, one of ligand-dependent transcription factors. Although avian and mammalian progesterone receptors (PR) have been well characterized, detail structure and function of amphibian progesterone receptor in wild frog is poorly studied yet. Here we report the cloning and characterization of a novel progesterone receptor from the Korean frog, Rana dybowskii. The R. dybowskii progesterone receptor (dyPR, GenBank Accession No. AF431813) cDNA isolated from testis encodes a protein of 711 amino acids which shows approximately 60% overall identity with the Xenopus progesterone receptor. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrates that dyPR is expressed in all the tissues examined. Electrophoretic mobility shift assays demonstrate that this receptor specifically binds to a progesterone response element (PRE), and transient transfection studies demonstrate that dyPR significantly activates the transcription of a PRE containing reporter element. Finally, confocal microscopy demonstrates the localization of this protein in nucleus, cytoplasm, and plasma membrane in transiently transfected CV-1 cell. These results indicate that dyPR cDNA encodes a classical progesterone receptor and molecular characterization of dyPR may provide us new information about the evolution of steroid hormone receptor.

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume135
Issue number1
DOIs
StatePublished - Jan 1 2004

Fingerprint

Ranidae
Molecular Cloning
Rana
Amphibians
Progesterone Receptors
amphibians
molecular cloning
Progesterone
progesterone
response elements
Response Elements
Anura
frogs
Complementary DNA
Steroid Receptors
Nucleic Acid Databases
hormone receptors
Xenopus
Electrophoretic Mobility Shift Assay
transfection

Keywords

  • dyPR
  • Frog
  • Nuclear receptor
  • Progesterone receptor

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

Cite this

Molecular cloning and characterization of an amphibian progesterone receptor from Rana dybowskii. / Wang, Li; Sanyal, Sabyasachi; Oh, Dayoung; Kim, Joon Young; Ju, Jung Won; Song, Kwang Hoon; Kim, Jung Woo; Kwon, Hyuk Bang; Choi, Hueng Sik.

In: General and Comparative Endocrinology, Vol. 135, No. 1, 01.01.2004, p. 142-149.

Research output: Contribution to journalArticle

Wang, Li ; Sanyal, Sabyasachi ; Oh, Dayoung ; Kim, Joon Young ; Ju, Jung Won ; Song, Kwang Hoon ; Kim, Jung Woo ; Kwon, Hyuk Bang ; Choi, Hueng Sik. / Molecular cloning and characterization of an amphibian progesterone receptor from Rana dybowskii. In: General and Comparative Endocrinology. 2004 ; Vol. 135, No. 1. pp. 142-149.
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