Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue

Lu Wang, Chunxiao Wu, Ying Zheng, Xiaoyan Qiu, Li Wang, Hui Fan, Wenling Han, Bingfeng Lv, Ying Wang, Xiaohui Zhu, Mingxu Xu, Peiguo Ding, Shanhong Cheng, Yingmei Zhang, Quansheng Song, Dalong Ma

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Chemokine-like factor (CKLF) was isolated from PHA-stimulated U937 cells. It is composed of 152 amino acids and located on chromosome 16q22. Utilizing bioinformatics, based on CKLF cDNA and protein sequences, in combination with experimental validation, we identified a novel gene designated chemokine-like factor super family member 1 (CKLFSF1). CKLFSF1 maps on chromosome 16q22, and the full-length gene comprises of seven exons and six introns. Using RACE-PCR, we identified two potential alternative transcription start sites, 1A and 1B. Northern blot and RT-PCR analysis demonstrated that CKLFSF1 is predominantly expressed in human testis tissue, with only lower levels of expression in many other human tissues. RT-PCR and cDNA sequencing identified 23 alternatively spliced isoforms of CKLFSF1 in the testis tissue, which encode protein variants ranging from 36 to 169 amino acids in length. Immunohistochemistry analysis demonstrated that CKLFSF1 proteins are highly expressed in spermatocyte and in tissue fluid of human testes tissue. In light of these findings, we propose that CKLFSF1 may play an important role in spermatogenesis or testicular development.

Original languageEnglish (US)
Pages (from-to)1492-1501
Number of pages10
JournalInternational Journal of Biochemistry and Cell Biology
Volume36
Issue number8
DOIs
StatePublished - Aug 2004

Fingerprint

Cloning
Alternative Splicing
Molecular Cloning
Chemokines
Testis
Protein Isoforms
Genes
Tissue
Chromosomes
Polymerase Chain Reaction
Complementary DNA
Amino Acids
U937 Cells
Proteins
Spermatocytes
Transcription Initiation Site
Spermatogenesis
Bioinformatics
Computational Biology
Northern Blotting

Keywords

  • base pair(s)
  • bp
  • cDNA
  • chemokine-like factor
  • chemokine-like factor super family member 1
  • CKLF
  • CKLFSF1
  • DNA complementary to RNA
  • double strand
  • ds
  • EST
  • expressed sequence tag
  • horseradish peroxidase
  • HRP
  • kb
  • kDa
  • kilobase(s) or 1000 bp
  • kilodalton(s)
  • nt

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue. / Wang, Lu; Wu, Chunxiao; Zheng, Ying; Qiu, Xiaoyan; Wang, Li; Fan, Hui; Han, Wenling; Lv, Bingfeng; Wang, Ying; Zhu, Xiaohui; Xu, Mingxu; Ding, Peiguo; Cheng, Shanhong; Zhang, Yingmei; Song, Quansheng; Ma, Dalong.

In: International Journal of Biochemistry and Cell Biology, Vol. 36, No. 8, 08.2004, p. 1492-1501.

Research output: Contribution to journalArticle

Wang, Lu ; Wu, Chunxiao ; Zheng, Ying ; Qiu, Xiaoyan ; Wang, Li ; Fan, Hui ; Han, Wenling ; Lv, Bingfeng ; Wang, Ying ; Zhu, Xiaohui ; Xu, Mingxu ; Ding, Peiguo ; Cheng, Shanhong ; Zhang, Yingmei ; Song, Quansheng ; Ma, Dalong. / Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue. In: International Journal of Biochemistry and Cell Biology. 2004 ; Vol. 36, No. 8. pp. 1492-1501.
@article{e3dad442c7914f69906af91c14bff25e,
title = "Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue",
abstract = "Chemokine-like factor (CKLF) was isolated from PHA-stimulated U937 cells. It is composed of 152 amino acids and located on chromosome 16q22. Utilizing bioinformatics, based on CKLF cDNA and protein sequences, in combination with experimental validation, we identified a novel gene designated chemokine-like factor super family member 1 (CKLFSF1). CKLFSF1 maps on chromosome 16q22, and the full-length gene comprises of seven exons and six introns. Using RACE-PCR, we identified two potential alternative transcription start sites, 1A and 1B. Northern blot and RT-PCR analysis demonstrated that CKLFSF1 is predominantly expressed in human testis tissue, with only lower levels of expression in many other human tissues. RT-PCR and cDNA sequencing identified 23 alternatively spliced isoforms of CKLFSF1 in the testis tissue, which encode protein variants ranging from 36 to 169 amino acids in length. Immunohistochemistry analysis demonstrated that CKLFSF1 proteins are highly expressed in spermatocyte and in tissue fluid of human testes tissue. In light of these findings, we propose that CKLFSF1 may play an important role in spermatogenesis or testicular development.",
keywords = "base pair(s), bp, cDNA, chemokine-like factor, chemokine-like factor super family member 1, CKLF, CKLFSF1, DNA complementary to RNA, double strand, ds, EST, expressed sequence tag, horseradish peroxidase, HRP, kb, kDa, kilobase(s) or 1000 bp, kilodalton(s), nt",
author = "Lu Wang and Chunxiao Wu and Ying Zheng and Xiaoyan Qiu and Li Wang and Hui Fan and Wenling Han and Bingfeng Lv and Ying Wang and Xiaohui Zhu and Mingxu Xu and Peiguo Ding and Shanhong Cheng and Yingmei Zhang and Quansheng Song and Dalong Ma",
year = "2004",
month = "8",
doi = "10.1016/j.biocel.2003.11.017",
language = "English (US)",
volume = "36",
pages = "1492--1501",
journal = "International Journal of Biochemistry and Cell Biology",
issn = "1357-2725",
publisher = "Elsevier Limited",
number = "8",

}

TY - JOUR

T1 - Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue

AU - Wang, Lu

AU - Wu, Chunxiao

AU - Zheng, Ying

AU - Qiu, Xiaoyan

AU - Wang, Li

AU - Fan, Hui

AU - Han, Wenling

AU - Lv, Bingfeng

AU - Wang, Ying

AU - Zhu, Xiaohui

AU - Xu, Mingxu

AU - Ding, Peiguo

AU - Cheng, Shanhong

AU - Zhang, Yingmei

AU - Song, Quansheng

AU - Ma, Dalong

PY - 2004/8

Y1 - 2004/8

N2 - Chemokine-like factor (CKLF) was isolated from PHA-stimulated U937 cells. It is composed of 152 amino acids and located on chromosome 16q22. Utilizing bioinformatics, based on CKLF cDNA and protein sequences, in combination with experimental validation, we identified a novel gene designated chemokine-like factor super family member 1 (CKLFSF1). CKLFSF1 maps on chromosome 16q22, and the full-length gene comprises of seven exons and six introns. Using RACE-PCR, we identified two potential alternative transcription start sites, 1A and 1B. Northern blot and RT-PCR analysis demonstrated that CKLFSF1 is predominantly expressed in human testis tissue, with only lower levels of expression in many other human tissues. RT-PCR and cDNA sequencing identified 23 alternatively spliced isoforms of CKLFSF1 in the testis tissue, which encode protein variants ranging from 36 to 169 amino acids in length. Immunohistochemistry analysis demonstrated that CKLFSF1 proteins are highly expressed in spermatocyte and in tissue fluid of human testes tissue. In light of these findings, we propose that CKLFSF1 may play an important role in spermatogenesis or testicular development.

AB - Chemokine-like factor (CKLF) was isolated from PHA-stimulated U937 cells. It is composed of 152 amino acids and located on chromosome 16q22. Utilizing bioinformatics, based on CKLF cDNA and protein sequences, in combination with experimental validation, we identified a novel gene designated chemokine-like factor super family member 1 (CKLFSF1). CKLFSF1 maps on chromosome 16q22, and the full-length gene comprises of seven exons and six introns. Using RACE-PCR, we identified two potential alternative transcription start sites, 1A and 1B. Northern blot and RT-PCR analysis demonstrated that CKLFSF1 is predominantly expressed in human testis tissue, with only lower levels of expression in many other human tissues. RT-PCR and cDNA sequencing identified 23 alternatively spliced isoforms of CKLFSF1 in the testis tissue, which encode protein variants ranging from 36 to 169 amino acids in length. Immunohistochemistry analysis demonstrated that CKLFSF1 proteins are highly expressed in spermatocyte and in tissue fluid of human testes tissue. In light of these findings, we propose that CKLFSF1 may play an important role in spermatogenesis or testicular development.

KW - base pair(s)

KW - bp

KW - cDNA

KW - chemokine-like factor

KW - chemokine-like factor super family member 1

KW - CKLF

KW - CKLFSF1

KW - DNA complementary to RNA

KW - double strand

KW - ds

KW - EST

KW - expressed sequence tag

KW - horseradish peroxidase

KW - HRP

KW - kb

KW - kDa

KW - kilobase(s) or 1000 bp

KW - kilodalton(s)

KW - nt

UR - http://www.scopus.com/inward/record.url?scp=2442502775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442502775&partnerID=8YFLogxK

U2 - 10.1016/j.biocel.2003.11.017

DO - 10.1016/j.biocel.2003.11.017

M3 - Article

C2 - 15147728

AN - SCOPUS:2442502775

VL - 36

SP - 1492

EP - 1501

JO - International Journal of Biochemistry and Cell Biology

JF - International Journal of Biochemistry and Cell Biology

SN - 1357-2725

IS - 8

ER -